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Traces of pandemic fluoroquinolone-resistant Escherichia coli clone ST131 transmitted from human society to aquatic environments and wildlife in Japan

Affiliations
  • 1Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
  • 2Graduate School of Infectious Diseases, Hokkaido University, Sapporo, Japan.
  • 3One Health Research Center, Hokkaido University, Sapporo, Japan.
  • 4Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 5Department of Infection Control and Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 6Department of Human Environmental Science, Graduate School of Human Development and Environment, Kobe University, Kobe, Japan.
  • 7Joint Department of Veterinary Medicine, Tottori University, Tottori, Japan.
  • 8The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
  • 9Division of Laboratory Medicine, Sapporo Medical University Hospital, Sapporo, Japan.

Published on:

July 16, 2024

Abstract

Transmission of antimicrobial-resistant bacteria among humans, animals, and the environment is a growing concern worldwide. The distribution of an international high-risk fluoroquinolone-resistant clone, ST131, has been documented in clinical settings. However, the transmission of ST131 from humans to surrounding environments remains poorly elucidated. To comprehend the current situation and identify the source of ST131 in nature, we analyzed the genetic features of ST131 isolates from the aquatic environment (lake/river water) and wildlife (fox, raccoon, raccoon dog, and deer) and compared them with the features of isolates from humans in Japan using accessory and core genome single nucleotide polymorphism (SNP) analyses. We identified ST131 isolates belonging to the same phylotype and genome clusters (four of eight clusters were concomitant) with low SNP distance between the human isolates and those from the aquatic environment and wildlife. These findings warn of ST131 transmission between humans and the surrounding environment in Japan.

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