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Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I

M Furchner1, A L Erickson, T Allen

  • 1Children's Research Institute, Children's Hospital, Inc., Department of Pediatrics, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio, USA.

Journal of Virology
|September 11, 1999
PubMed

Insights

Researchers identified three cytotoxic T lymphocyte (CTL) epitopes in simian immunodeficiency virus (SIV) proteins. These Mamu-A*01-restricted epitopes, found in the SIV envelope and Gag proteins, are crucial for monitoring immune responses in macaques.

Area of Science:

  • Immunology
  • Virology
  • Primate Models

Background:

  • Cytotoxic T lymphocyte (CTL) responses are critical for controlling viral infections, including simian immunodeficiency virus (SIV).
  • MHC class I alleles, such as Mamu-A*01, play a significant role in presenting viral epitopes to CTLs.
  • Identifying specific T cell epitopes is essential for developing effective vaccines and monitoring immune responses.

Purpose of the Study:

  • To identify and characterize CTL epitopes recognized during SIV infection in a Mamu-A*01-positive rhesus macaque.
  • To investigate the role of Mamu-A*01 restriction in CTL recognition of SIV envelope and Gag proteins.
  • To evaluate the utility of identified epitopes for monitoring immune responses.

Main Methods:

  • Monitoring CTL responses against SIV envelope (gp160) and Gag proteins in an infected rhesus macaque.
  • Culturing peripheral blood mononuclear cells (PBMC) with synthetic peptides spanning SIV proteins.
  • Assessing T cell recognition and identifying specific Mamu-A*01-restricted epitopes.

Main Results:

  • A total of three SIV epitopes were recognized by CTLs.
  • One epitope was located in the Gag protein (p11cC-->M), previously described as Mamu-A*01-restricted.
  • Two novel Mamu-A*01-restricted epitopes (p15m and p54m) were identified in the SIV gp160 envelope protein.
  • Mamu-A*01 expression was required for CD8+ T cell recognition of envelope-specific epitopes.

Conclusions:

  • Identified Mamu-A*01-restricted epitopes in SIV Gag and envelope proteins.
  • These epitopes are recognized by CTLs during SIV infection.
  • The identified SIV envelope epitopes will aid in monitoring immune responses in vaccinated or infected non-human primates.

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