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Updated: Jul 26, 2026
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Clinicopathologic, immunophenotypic, and molecular characterization of primary cutaneous follicular B-cell lymphoma
R Bergman1, P J Kurtin, L E Gibson
1Department of Dermatology, Rambam Medical Center, POB 9602, 31096 Haifa, Israel. dermatology@rambam.health.gov.il
Insights
Primary follicular cutaneous B-cell lymphoma (CBCL) is common and indolent. It shares features with nodal lymphoma but has lower bcl-2 expression and gene rearrangements.
Area of Science:
- Hematology
- Oncology
- Dermatopathology
Background:
- Follicle center lymphoma-follicular (FCL-F) is a subtype of primary cutaneous B-cell lymphoma (CBCL).
- Understanding its characteristics is crucial for accurate diagnosis and treatment.
Purpose of the Study:
- To define the clinicopathologic, immunophenotypic, and molecular features of primary FCL-F CBCL.
- To compare these features with primary nodal FCL-F.
Main Methods:
- Retrospective analysis of medical records and archival biopsy specimens from 21 primary FCL-F CBCL patients (1987-1997).
- Immunohistochemical studies for B- and T-cell lineages, CD10, bcl-2, CD43, and cytoplasmic immunoglobulin light chains.
- Molecular studies including Ig heavy-chain and bcl-2 gene rearrangements.
Main Results:
- The head and neck were the most frequent primary sites.
- Recurrences were common, but the overall mortality rate was low (4.8%) over a mean follow-up of 6.3 years.
- While Ig heavy-chain gene rearrangements indicated clonality in 94% of cases, bcl-2 gene rearrangements were significantly lower in primary cutaneous FCL-F (13%) compared to primary nodal FCL-F (75%).
Conclusions:
- Primary cutaneous FCL-F is a common, indolent CBCL subtype.
- It shares many characteristics with primary nodal FCL-F.
- Key differences include significantly lower rates of bcl-2 expression and gene rearrangements in the cutaneous form.
Objective:
To determine the clinicopathologic, immunophenotypic, and molecular characteristics of primary follicular cutaneous B-cell lymphoma (CBCL) as defined by the revised European-American lymphoma classification.
Design:
A retrospective survey of the medical records, an immunohistochemical study of archival biopsy specimens. and molecular studies of preserved DNA of all patients with follicle center lymphoma-follicular (FCL-F) primary CBCL from 1987 to 1997.
Setting:
A single-center outpatient specialty clinic at an academic medical center.
Patients:
Twenty-one patients (68% of all new primary CBCL cases), including 14 men and 7 women (age range, 33-88 years; mean, 55 years).
Results:
The head and neck region was the most frequent primary site. Following treatment, recurrences were relatively frequent, but the overall mortality rate during 1.0 to 11.3 years (mean, 6.3 years) of follow-up was 4.8%. Immunohistochemical analysis for B- and T-cell lineages was helpful in enhancing the folliclelike structures. CD10, bcl-2, and CD43 were expressed by the neoplastic cells in 9 (47%) of 19 cases, 4 (21%) of 19 cases, and 2 (13%) of 16 cases, respectively. Immunohistochemical detection of cytoplasmic immunoglobulin light chains, using steaming in EDTA as the antigen-retrieval technique, was successful in 12 (71%) of 17 cases. The Ig heavy-chain gene rearrangements, using the Southern blot technique, detected clonality in 17 (94%) of 18 cases. The bcl-2 gene rearrangements were detected in only 2 (13%) of 15 of the primary cutaneous FCL-F cases, compared with 9 (75%) of 12 of the primary nodal FCL-F cases (P =.002).
Conclusions:
Primary cutaneous FCL-F is a relatively common subtype of CBCL, with a relatively indolent course. It has many features in common with primary nodal FCL-F, except for low rates of bcl-2 expression and bcl-2 gene rearrangements.
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