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Published on: November 22, 2013
Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14
1Department of Microbiology and Immunology, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Aichi, Japan.
Insights
Membrane-bound CD14 enhances B1 cell sensitivity to lipopolysaccharide (LPS). This molecule is crucial for LPS recognition, boosting DNA synthesis and signaling pathways in mouse B1 cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- B1 cells are a distinct subset of lymphocytes.
- Lipopolysaccharide (LPS) is a key component of Gram-negative bacteria recognized by the innate immune system.
- CD14 is a pattern recognition receptor involved in LPS sensing.
Purpose of the Study:
- To investigate the role of membrane-bound CD14 in the response of mouse B1 cell lines to LPS.
- To determine if CD14 expression influences LPS sensitivity in B1 cells.
Main Methods:
- Characterization of mouse TH2.52 B cell surface markers to confirm B1 cell phenotype.
- Assessment of TH2.52 B1 cell response to LPS, including DNA synthesis, NF-kappaB activation, and Erk1/2 phosphorylation.
- Inhibition studies using anti-CD14 antibody.
Main Results:
- TH2.52 B1 cells expressed typical B1 cell markers and membrane-bound CD14.
- These cells exhibited heightened sensitivity to low LPS concentrations, showing increased DNA synthesis and activation of NF-kappaB and Erk1/2 pathways.
- Anti-CD14 antibody significantly inhibited the LPS response.
Conclusions:
- Membrane-bound CD14 expression confers high sensitivity to LPS in mouse B1 cells.
- CD14 is essential for mediating LPS recognition and subsequent cellular activation in this B1 cell line.
Abstract:
The role of membrane-bound CD14 in the response of mouse B1 cell lines to lipopolysaccharide (LPS) was studied. The surface profile of mouse TH2.52 B cells was positive for CD5, IgM, B220, CD11b and F4/80, suggesting that TH2.52 cells carried the typical phenotype of B1 cells. Furthermore, TH2.52 B1 cells were found to express membrane-bound CD14, which plays a critical role in LPS recognition. TH2.52 B1 cells responded to a very low concentration of LPS and exhibited: (i) augmentation of DNA synthesis; (ii) activation of nuclear factor (NF)-kappaB; and (iii) phosphorylation of extracellular signal regulated kinase 1/2 (Erk1/2). They were markedly inhibited by anti-CD14 antibody. Therefore, the expression of membrane-bound CD14 was suggested to provide high sensitivity to LPS for TH2.52 B1 cells.
