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[Histological study of conjunctiva-associated lymphoid tissue in mice]
Tohru Sakimoto1, Jun Shoji, Keiko Saito
1Department of Ophthalmology, Nihon University School of Medicine, 30-1 Ooyaguchikami-machi, Itabashi-ku, Tokyo 173-8610, Japan.
Insights
Conjunctiva-associated lymphoid tissue (CALT) was identified in mouse nictitating membranes. Topical ovalbumin treatment increased this follicular tissue, confirming its presence and immune cell infiltration in mice.
Area of Science:
- Immunology
- Ophthalmology
- Histology
Background:
- Conjunctiva-associated lymphoid tissue (CALT) plays a crucial role in ocular immune responses.
- Understanding CALT's structure and function is vital for ocular immunology research.
Purpose of the Study:
- To investigate the presence and characteristics of conjunctiva-associated lymphoid tissue (CALT) in mouse conjunctiva.
- To determine if follicular tissue in the mouse conjunctiva represents CALT.
Main Methods:
- Histological examination of conjunctival tissue in normal mice (4-6 weeks old).
- Topical instillation of ovalbumin and cholera toxin B in mice.
- Immunohistological analysis of conjunctival follicles and intraepithelial pockets.
Main Results:
- Follicular tissue was identified histologically at the nictitating membrane in mice.
- Topical ovalbumin treatment led to an increase in the size and amount of this follicular tissue.
- Immunohistochemistry revealed CD4+, CD8+, and S-100 protein-positive cells within the follicular tissue and intraepithelial pockets.
Conclusions:
- The follicular tissue located at the nictitating membrane of mice is identified as conjunctiva-associated lymphoid tissue (CALT).
- This finding establishes a model for studying ocular immune responses in mice.
Purpose:
To investigate conjunctiva-associated lymphoid tissue (CALT) in mouse conjunctiva.
Method:
We examined normal mice, ranging in age from 4 to 6 weeks, to investigate the presence of follicular tissue histologically. Next, we treated mice by topical instillation of ovalbumin and cholera toxin B, and then examined conjunctival follicles by immunohistological methods.
Results:
Follicular tissue was present at the nictitating membrane. Both size and amount of follicular tissue was increased by the topical ovalbumin treatment. Immunohistological examination revealed CD4, CD8, and S-100 protein positive cells in follicular tissue. The epithelial layer, corresponding to follicular tissue, had intraepithelial pockets and CD4 positive cells in the pockets.
Conclusion:
It was concluded that the follicular tissue at the nictitating membrane is CALT in mice.