Related Experiment Video

Updated: Aug 11, 2026

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Subpopulation composition of peripheral blood lymphocytes in children with infectious mononucleosis

V V Novitskii1, O I Urazova, I O Naslednikova

  • 1Department of Pathophysiology, Siberian State Medical University, Tomsk.

Bulletin of Experimental Biology and Medicine
|December 3, 2002
PubMed

Insights

Infectious mononucleosis, both viral and bacterial, elevates CD72 and CD16 lymphocyte levels. Viral cases also show persistent CD8 cell increases post-recovery, unlike bacterial cases where CD72 levels normalize.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Hematology

Background:

  • Infectious mononucleosis is a common illness caused by Epstein-Barr virus (EBV) or other viruses, and less commonly by bacteria.
  • Immune system responses, particularly lymphocyte populations, are crucial in managing viral and bacterial infections.
  • Understanding lymphocyte dynamics in infectious mononucleosis can provide insights into disease pathogenesis and recovery.

Purpose of the Study:

  • To investigate the changes in specific lymphocyte subsets (CD72+, CD16+, CD8+) during acute and convalescent phases of viral and bacterial infectious mononucleosis.
  • To compare the recovery patterns of these lymphocyte populations between viral and bacterial forms of the disease.

Main Methods:

  • Blood samples were collected from patients during the acute and convalescent periods of infectious mononucleosis.
  • Flow cytometry was used to quantify the percentages of CD72+, CD16+, and CD8+ lymphocytes.
  • Comparison was made with healthy control groups.

Main Results:

  • Both viral and bacterial infectious mononucleosis showed increased levels of CD72+ and CD16+ lymphocytes during acute and convalescent phases compared to normal controls.
  • Patients with viral infectious mononucleosis exhibited an increase in CD8+ cells during convalescence, with these changes persisting into delayed recovery periods.
  • In bacterial infectious mononucleosis, CD72+ lymphocyte levels returned to normal approximately 18 months after convalescence.

Conclusions:

  • Infectious mononucleosis, regardless of etiology (viral or bacterial), is associated with significant alterations in CD72+ and CD16+ lymphocyte counts.
  • Viral infectious mononucleosis is characterized by a prolonged elevation of CD8+ lymphocytes, suggesting a distinct immune response compared to bacterial forms.
  • The normalization of CD72+ lymphocytes in bacterial infectious mononucleosis occurs over a longer timeframe, highlighting differences in immune system recovery.

More Related Videos

Selective Harvesting of Marginating-pulmonary Leukocytes07:06

Selective Harvesting of Marginating-pulmonary Leukocytes

Published on: March 11, 2016

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Related Concept Videos

Structure and Function of Leukocytes01:21

Structure and Function of Leukocytes

An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
White blood cells protect the body...
Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...