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Published on: July 31, 2012
Expression of the Protein with Molecular Weight 104-110 kDa by Normal and Malignant Human B Lymphoid Cells
Serguei Kisselev1, Vladimir Kozhevnikov
1Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk, Russia.
Insights
A novel cell surface protein, p104-110, is identified on B lymphoid cells and certain malignant B cell populations. Its expression is investigated in relation to B cell differentiation and activation.
Area of Science:
- Immunology
- Cell Biology
- Hematology
Background:
- A novel cell surface protein, p104-110, has been detected using a laboratory-developed monoclonal antibody.
- The expression pattern of p104-110 across various blood cell types is not fully understood.
Purpose of the Study:
- To characterize the distribution of the p104-110 cell surface protein.
- To investigate the association of p104-110 expression with B cell differentiation and activation.
Main Methods:
- Monoclonal antibody production for p104-110 detection.
- Flow cytometry (FACS) analysis of peripheral blood mononuclear cells and bone marrow cells.
- Analysis of cell lines and patient samples including chronic lymphoid leukemia and non-Hodgkin's lymphoma.
Main Results:
- p104-110 is expressed on B lymphoid cell lines but not other blood lineages.
- In healthy donors, p104-110 is found on a minor population of peripheral blood mononuclear cells, present on both CD20(+) and CD20(-) subpopulations.
- p104-110 is detected on CD34(-); CD38(+) mononuclear cells in bone marrow and highly expressed on malignant CD19(+)CD5(+)CD20(-) chronic lymphoid leukemia cells, with increased expression in some non-Hodgkin's lymphomas.
Conclusions:
- p104-110 is a potential marker for specific B cell populations.
- Expression of p104-110 may correlate with B cell differentiation stages and activation states.
- Further research into p104-110's role in B cell biology and disease is warranted.
Abstract:
In this paper we present the results of the distribution of a cell surface protein with molecular weight 104-110 kDa (p104-110) detected by monoclonal antibody made in our laboratory. The protein is expressed by a number of cell lines belonging to B lymphoid cells, but not to the other blood cell lineages. In healthy donors the expression of p104-110 is restricted to a minor population of human peripheral blood mononuclear cells shared by both CD20(+) and CD20(-) subpopulation. No expression of p104-110 has been found on resting and activated T cells as well as on monocytes and polymorphonuclear cells. FACS analysis of mononuclear cells derived from normal human bone marrow has revealed the presence of p104-110 on committed CD34(-); CD38(+) mononuclear cell subset that composes only the minor part of mature CD20(+) B cells being mostly CD20 negative. Furthermore, CD19(+)CD5(+)CD20(-) malignant cells derived from the patients with chronic lymphoid leukemia express p104-110 at high level. Similarly, it has been shown that some patients with malignant non-Hodgkin's lymphomas can have the increased contents of CD20(+) cells bearing that surface antigen. Possible associations of p104-110 expression on human B lymphoid cells with their differentiation and activation are discussed.
