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Acidic alpha-D-mannosidase in phenotypically different leukemic lymphoid cells
N A Ushakova1, R S Samojlova, M E Preobrazhenskaya
1Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
Insights
Acid alpha-mannosidase activity varies in lymphoid cells from patients with lymphoproliferative disorders. This enzyme
Area of Science:
- Biochemistry
- Immunology
- Hematology
Background:
- Acid alpha-mannosidase is an enzyme with a role in cellular processes.
- Lymphoproliferative disorders involve abnormal lymphocyte proliferation.
- Understanding enzyme activity in these cells can aid diagnosis.
Purpose of the Study:
- To investigate acid alpha-mannosidase activity in lymphoid cells from patients with lymphoproliferative disorders.
- To correlate enzyme activity with specific cell immunophenotypes and disease types.
- To explore the diagnostic potential of acid alpha-mannosidase activity in hematopoietic malignancies.
Main Methods:
- Phenotypic characterization of lymphoid cells from peripheral blood, spleen, and lymph nodes.
- Isolation of cells based on immunophenotype.
- Assay of acid alpha-mannosidase enzyme activity in isolated cell populations.
Main Results:
- Significant variations in acid alpha-mannosidase activity were observed across different lymphoid cell types.
- Lowest activity was found in early B cells from B-cell chronic lymphocytic leukemia (B-CLL) patients.
- Highest activity was detected in activated, CD11c-expressing B cells from B-cell non-Hodgkin lymphoma (B-NHL) and hairy cell leukemia (HCL) patients.
- Differences in enzyme activity were noted between peripheral blood and spleen cells in B-NHL patients with spleen involvement.
Conclusions:
- Acid alpha-mannosidase activity is a potential biomarker for classifying and diagnosing lymphoproliferative disorders.
- Enzyme activity levels correlate with specific immunophenotypes and disease subtypes.
- These findings may contribute to improved staging and management strategies for hematopoietic malignancies.
Abstract:
The activity of acid alpha-mannosidase in phenotypically characterized lymphoid cells, isolated from peripheral blood, spleen and lymph nodes of patients with various lymphoproliferative disorders has been studied. Cells with different immunophenotypes were shown to have different alpha-mannosidase activity levels. The lowest alpha-mannosidase activity was observed in cells phenotypically corresponding to early B cells obtained from B-CLL patients. The highest activity was determined in cells with phenotypes of activated, CD11c-expressing B cells from B-NHL and HCL patients. There were considerable differences in alpha-mannosidase activity between peripheral blood and spleen lymphoid cells of B-NHL patients with spleen damage. The data obtained may be used in classification, primary diagnosis and staging of hematopoietic malignancies.