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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

[The morphological and immunological characteristics of less-differentiated acute myeloid leukemic cells]

Yi-Yan Lai1, Huo Tan, Xu Ye

  • 1Department of Hematology, The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China. cq2@21cn.com

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|September 10, 2003
PubMed

Insights

Diagnosing acute myeloid leukemia (AML-Mo) relies on morphology, histochemistry, and immunophenotyping of bone marrow cells. Specific staining patterns and flow cytometry markers aid in accurate AML classification.

Area of Science:

  • Hematology
  • Oncology
  • Cell Biology

Background:

  • Accurate classification of acute myeloid leukemia (AML) is crucial for effective treatment.
  • Less-differentiated subtypes, such as AML with monocytic differentiation (AML-Mo), present diagnostic challenges.
  • Morphological, histochemical, and immunological features are key diagnostic parameters.

Observation:

  • Bone marrow smears from two AML-Mo cases were analyzed using Wright-Giemsa and histochemical stains.
  • Immunophenotyping was performed using flow cytometry to determine cell surface markers.
  • Key observations included negative myeloperoxidase staining, positive periodic acid-Schiff (PAS) staining with fine particles, and positive expression of CD33/CD13, while CD2/CD3/CD10/CD19/CD22 were negative.

Findings:

  • The study identified specific morphological, histochemical, and immunophenotypic profiles for AML-Mo.
  • Negative myeloperoxidase and positive PAS staining, along with CD33/CD13 expression, are characteristic.
  • The combined analysis of these markers provides a robust diagnostic basis for AML-Mo.

Implications:

  • Morphology, histochemistry, and immunophenotyping of bone marrow are essential for diagnosing AML-Mo.
  • Utilizing a panel of monoclonal antibodies in flow cytometry enhances diagnostic accuracy for AML subtypes.
  • This approach aids in precise patient stratification and personalized therapeutic strategies for AML.

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