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Published on: May 4, 2012
Human centrosomal epitope is shared specifically with human lactate dehydrogenase-B isozyme
1Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.
Insights
Rabbit serum 0013 identifies a specific epitope on human lactate dehydrogenase B (LDH-B) subunits. This finding provides the most potent anti-LDH-B serum available for research.
Area of Science:
- Cell Biology
- Biochemistry
- Immunology
Background:
- Rabbit serum 0013 was initially used to identify centrosomal proteins.
- The serum also recognized a 36 kDa non-centrosomal protein, identified as human lactate dehydrogenase (LDH).
- The co-distribution of the 0013 epitope on centrosomal proteins and LDH was found to be specific to human cells, with human hepatic cells being an exception.
Purpose of the Study:
- To investigate the specificity of the 0013 epitope in human cells.
- To determine the precise target of the 0013 epitope within lactate dehydrogenase.
- To characterize the anti-LDH-B activity of serum 0013.
Main Methods:
- Immunological assays using rabbit serum 0013.
- Analysis of human hepatic cells expressing only the LDH-A4 isozyme.
- Characterization of lactate dehydrogenase subunits.
Main Results:
- The 0013 epitope is specific for the human LDH-B subunit.
- Serum 0013 demonstrates high specificity for LDH-B.
- Human hepatic cells, expressing only LDH-A4, confirmed the epitope's specificity for LDH-B.
Conclusions:
- Serum 0013 is a highly specific reagent for detecting the human LDH-B subunit.
- This specificity offers a valuable tool for studying LDH-B in various biological contexts.
- The findings prompt further investigation into the evolutionary and physiological significance of LDH-B specificity.
Abstract:
A rabbit serum (0013) used to identify pericentriolar proteins from isolated centrosomes (Gosti-Testu, F., Marty, M.C., Berges, J., Maunoury, R. and Bornens, M. (1986) EMBO J. 5, 2545-2550) was shown also to react through the same epitope with several non-centrosomal proteins including a major 36 kDa cytosolic antigen. This protein was identified to be human lactate dehydrogenase and the co-distribution of 0013 epitope on the centrosomal protein and on lactate dehydrogenase (LDH) was shown to be specific for human cells (Gosti, F., Marty, M.C., Courvalin, J.C., Maunoury, R. and Bornens, M. (1987) Proc. Natl. Acad. Sci. USA 84, 1000-1004). Human hepatic cells constitute, so far, the only exception to this co-distribution rule. By using this cell type which expresses only the LDH-A4 isozyme, we demonstrate that 0013 epitope is specific for the human LDH-B subunit, making serum 0013 the strongest anti-LDH-B available so far. The evolutionary and physiological significance of this situation is discussed.
