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Published on: July 11, 2015
Non-specific natural cytotoxic factor released from bovine peripheral blood lymphocytes
T Kosaka1, M Kuwabara, F Koide
1Department of Veterinary Radiology, School of Veterinary Medicine, Nihon University, Kanagawa, Japan.
Insights
Natural cytotoxic cells (NCC) in bovine peripheral blood lymphocytes exhibit potent cytotoxicity against bovine leukemia cells. These cells release a natural cytotoxic factor (NCF) that mediates this activity, with efficacy increasing over incubation time.
Area of Science:
- Immunology
- Cell Biology
- Veterinary Science
Background:
- Bovine leukemia cells (PC-3) are susceptible to natural cytotoxicity.
- Natural cytotoxic cells (NCC) are found in T cell-rich fractions of bovine peripheral blood lymphocytes (PBL).
Purpose of the Study:
- To investigate the role of bovine peripheral blood lymphocytes (PBL) in natural cytotoxicity against bovine leukemia cells (PC-3).
- To characterize the natural cytotoxic factor (NCF) released by natural cytotoxic cells (NCC).
Main Methods:
- Isolation of non-adherent cells from bovine PBL using nylon-wool columns.
- Co-culture of natural cytotoxic cells (NCC) with PC-3 cells.
- Measurement of natural cytotoxic factor (NCF) release and activity.
- Assessment of cytotoxicity against various cell lines (PC-3, K562, CL-1, M1, EL-4).
Main Results:
- Natural cytotoxicity against PC-3 cells was primarily mediated by non-adherent cells (NCC) in bovine PBL.
- NCC rapidly released natural cytotoxic factor (NCF) upon co-culture with PC-3 cells.
- Cytotoxicity by NCC and NCF against PC-3 cells increased with incubation time.
- NCF demonstrated broad cytotoxicity against multiple cell lines, while NCC showed specific activity against PC-3 cells.
Conclusions:
- Bovine natural cytotoxic cells (NCC) play a significant role in eliminating leukemia cells.
- The released natural cytotoxic factor (NCF) is a key mediator of this anti-leukemia activity.
- NCF exhibits a distinct activity profile compared to NCC, suggesting specific mechanisms of action.
Abstract:
Natural cytotoxicity against bovine leukemia cells (PC-3 cells) was found in bovine peripheral blood lymphocytes (PBL), and in non-adherent cells but not in adherent cells to nylon-wool column. Natural cytotoxic cells (NCC), which have natural cytotoxic activity, are found in T cell-rich fraction. When NCC were cocultured with PC-3 cells, natural cytotoxic factor (NCF) was released rapidly from NCC, and dose-response curve for NCF was almost linear induction. Cytotoxicity against PC-3 cells by NCC or NCF was increased with an increment of incubation period. Cytotoxicity against K562 cells, CL-1 cells, M1 cells or EL-4 cells by NCF was almost the same level as that against PC-3 cells, but that against those cell lines by NCC was not found. NCF activity in culture fluid from NCC cocultured with K562 cells or CL-1 cells was lower than that from NCC cocultured with PC-3 cells.
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