Method development for determining the iohexol in human serum by micellar electrokinetic capillary chromatography

Area of Science:

• Analytical Chemistry
• Clinical Chemistry
• Biomedical Engineering

Background:

• Iohexol is a common non-ionic radiographic contrast medium used in clinical settings.
• Monitoring iohexol levels in blood is crucial for preventing adverse effects from systemic retention.
• Accurate and efficient determination methods are needed for clinical laboratory diagnostics.

Purpose of the Study:

• To develop a simplified and precise method for determining iohexol concentration in serum.
• To establish a no-pretreatment analytical technique for routine clinical use.
• To validate the performance characteristics of the developed method.

Main Methods:

• Micellar electrokinetic capillary chromatography (MECC) was employed for direct serum sample analysis.
• Electrophoresis was conducted at 25 kV using a borate buffer with sodium dodecyl sulfate.
• Detection was performed at 245 nm, with iohexol migration time recorded at 7.4 minutes.

Main Results:

• The method demonstrated good linearity from 0-1000 mg/l with a detection limit of 0.5 mg/l.
• Intra-assay precision showed coefficients of variation (CV) of 1.38-4.68% (6.2-200.1 mg/l range).
• Inter-assay precision yielded CVs of 2.06-5.94% (10.3-155.4 mg/l range), with recovery rates of 96-102%.

Conclusions:

• A novel MECC method allows for direct serum injection, significantly simplifying the determination procedure.
• The developed method offers high precision and accuracy for iohexol quantification in clinical samples.
• This technique facilitates timely monitoring of iohexol levels, contributing to patient safety and effective treatment.