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Published on: April 9, 2013
[Chronic idiopathic neutropenia. A cytofluorimetric method to detect antineutrophil antibodies]
Giovanni Carulli1, Massimo Ghimenti, Francesco Volpi
1Divisione di Ematologia, Dipartimento di Oncologia, Trapianti e delle Nuove Tecnologie in Medicina, Università, Pisa.
Insights
A cytofluorimetric method detected antineutrophil antibodies in 25% of adults with chronic idiopathic neutropenia. These antibodies were more prevalent in severe and moderate neutropenia cases.
Area of Science:
- Immunology
- Hematology
Background:
- Chronic idiopathic neutropenia is a condition characterized by low neutrophil counts.
- Antineutrophil antibodies are implicated in the pathogenesis of neutropenia.
Purpose of the Study:
- To investigate the prevalence of antineutrophil antibodies in adults with chronic idiopathic neutropenia using a cytofluorimetric method.
Main Methods:
- A cytofluorimetric assay was employed to detect antineutrophil antibodies.
- Both direct and indirect tests were performed on whole blood samples.
- F(ab')2 goat anti-human FITC-conjugates immunoglobulins were utilized.
Main Results:
- Antineutrophil antibodies were detected in 15 out of 60 (25%) adult patients.
- Antibodies were found on the neutrophil surface and in serum.
- Positive cases were more frequent in patients with severe (4/5) and moderate (8/16) neutropenia.
Conclusions:
- Cytofluorimetry is an effective method for detecting antineutrophil antibodies in chronic idiopathic neutropenia.
- A significant proportion of patients with chronic idiopathic neutropenia possess antineutrophil antibodies.
- The presence of these antibodies correlates with disease severity.
Abstract:
A cytofluorimetric method to detect antineutrophil antibodies was carried out in sixty adults suffering from chronic idiopathic neutropenia (5 patients with severe neutropenia, 16 with moderate neutropenia, 39 with mild neutropenia). Both the direct and the indirect tests were carried out using F(ab')2 goat anti-human FITC-conjugates immunoglobulins on whole blood samples. Fifteen patients (25%) were found to have antineutrophil antibodies both on the neutrophil surface and in serum. Most positive patients were suffering from severe neutropenia (4 patients) and moderate neutropenia (8 patients). Only four positive patients were suffering from infections.
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