Related Experiment Video
Updated: Aug 8, 2026
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
Published on: April 3, 2011
Canine lymphomas: a morphological and immunohistochemical study of 55 cases, with observations on p53
F A R Sueiro1, A C Alessi, J Vassallo
1Department of Anatomical Pathology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), São Paulo, P. O. Box 6111, 13081-970 Campinas, SP, Brazil.
Insights
This study characterized canine lymphomas (CLs) by immunophenotype and p53 protein expression. High-grade lymphomas and older patients showed increased p53 positivity, offering insights comparable to human lymphoma research.
Area of Science:
- Veterinary Pathology
- Immunohistochemistry
- Canine Oncology
Background:
- Canine lymphomas (CLs) require precise classification for effective treatment.
- Understanding the immunophenotype and molecular markers like p53 is crucial for prognosis.
Purpose of the Study:
- To determine the immunophenotype of canine lymphomas using WHO classification.
- To correlate immunophenotype with p53 protein immunoexpression.
- To compare findings with human lymphoma data.
Main Methods:
- Immunophenotyping of 55 canine lymphoma cases using antibodies for CD79a and CD3.
- Paraffin-embedded tissue sections were used for immunohistochemistry.
- p53 protein expression was assessed via immunolabeling.
Main Results:
- The majority of canine lymphomas were B-cell (72.7%) followed by T-cell (21.8%).
- p53 positivity was observed in 24 B-cell and 7 T-cell lymphomas.
- High-grade lymphomas and older patients showed significantly higher p53 positivity.
Conclusions:
- Immunophenotyping aligns with WHO classification for canine lymphomas.
- Increased p53 expression in high-grade CLs and older patients suggests a role in pathogenesis.
- Canine lymphoma findings parallel those in human lymphomas, supporting comparative oncology studies.
Abstract:
The purpose was to determine the immunophenotype of canine lymphomas (CLs) classified according to the WHO nomenclature for domestic animals, and to relate these findings to the immunoexpression of p53 protein. Lymphomas were immunophenotyped with antibodies to CD79a, CD3, and p53 protein, suitable for paraffin wax-embedded tissue sections. Of 55 cases, 40 (72.7%) were of the B-cell phenotype, 12 (21.8%) of the T-cell phenotype, and three (5.4%) were non-B-non-T lymphomas. Of the 40 B-cell lymphomas, 31 were of the large B-cell type, six were lymphoplasmacytic, one lymphocytic, one follicular (grade II) and one unclassified low-grade. One of the peripheral T-cell lymphomas was morphologically consistent with anaplastic large cell lymphoma. Immunolabelling for p53 occurred in 24 B-cell and seven T-cell lymphomas. Lymphomas with high grade histology showed a significantly increased frequency of p53 positivity (P = 0.01). Positivity for p53 (more than 10% positive cells) tended to be associated with the T-cell phenotype (P = 0.06). Mean patient age was significantly higher in p53-positive cases (P = 0.02). These data are comparable with findings in human lymphomas.
