Related Experiment Video
Updated: Aug 17, 2026
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
[A clinicopathological study of 96 cases of lymphoblastic lymphoma]
Yun Pan1, Wei-Ping Liu, Jin-Fan Li
1West China Hospital, Sichuan University, Chengdu 610041, China.
Insights
This study analyzed 96 lymphoblastic lymphoma (LBL) cases, finding T-cell LBL more common in young males with mediastinal masses. Prognosis depends on clinical stage, immunophenotype, and Ki-67 expression.
Area of Science:
- Oncology
- Hematopathology
- Immunohistochemistry
Background:
- Lymphoblastic lymphoma (LBL) is an aggressive non-Hodgkin lymphoma.
- Understanding its clinicopathological and immunohistochemical features is crucial for diagnosis and prognosis.
Purpose of the Study:
- To investigate the clinicopathological and immunohistochemical features of lymphoblastic lymphoma (LBL).
Main Methods:
- Retrospective study of 96 LBL cases.
- Immunohistochemical staining for characterization and immunophenotyping.
Main Results:
- Median age was 16 years; 69 males, 27 females.
- Mediastinal masses and lymphadenopathy were common.
- TdT (75.0%) and CD99 (92.7%) positivity observed.
- T-cell markers (78 cases) predominated over B-cell markers (18 cases).
- Younger patients (<30 years) and those with mediastinal masses showed higher T-LBL incidence.
- Poor prognostic factors included T-cell tumors, advanced stages, low Ki-67, and no chemotherapy.
Conclusions:
- Mediastinal masses with lymphadenopathy in young males suggest LBL.
- Negative TdT does not exclude LBL.
- T-LBL is more prevalent than B-LBL.
- Prognosis is linked to clinical stage, immunophenotype, and Ki-67 levels.
Objective:
To investigate the clinicopathological and immunohistochemical features of lymphoblastic lymphoma (LBL).
Methods:
A retrospective clinicopathological study of 96 cases LBL was carried out. Immunohistochemical staining was used for the characterization and immunophenotyping.
Results:
The patients age ranged from 4 to 72 years, with a median of 16 years, 69 patients were male and 27 female. Seventy-three cases had superficial or multi-lymphoadenopathy and 31 of them had mediastinal masses. Bone marrow was involved in 15 cases. Seventy-three cases were in clinical stages III and IV. The median survival of the followed-up patients was 5.5 (2 approximately 120) months. TdT and CD99 positive reactions were 75.0% and 92.7%, respectively. Of the 96 cases, 78 displayed T-cell marker positivity and 18 B-cell markers. 82.1% of the patients younger than 30 years of age had significantly higher incidences of T-LBL (64 patients), and 93.6% of the patients with mediastinal masses expressed T-cell markers. The poor prognostic factors were T-cell tumors, clinical stages III and IV, Ki-67 PI < 80% and no chemotherapy (P < 0.01).
Conclusion:
In children and young males, mediastinal masses with superficial or multi-lymphoadenopathy favors the diagnosis of LBL, but negative TdT reaction can not exclude this diagnosis. T-LBL is more common than B-LBL. Clinical stages, immunophenotypes and the level of Ki-67 expression were closely related with prognosis of LBL.