Related Experiment Video
Updated: Aug 13, 2026
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
Published on: October 18, 2016
Identification of bovine dendritic cell phenotype from bovine peripheral blood
Kohtaro Miyazawa1, Hisashi Aso, Masashi Honda
1Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan.
Insights
Researchers identified a new phenotype for bovine dendritic cells (DCs) in peripheral blood, crucial for understanding immune responses and tolerance in cattle. This finding aids in developing methods for isolating and characterizing these important immune cells.
Area of Science:
- Immunology
- Veterinary Science
- Cell Biology
Background:
- Dendritic cells (DCs) are critical antigen-presenting cells for initiating immune responses and peripheral tolerance.
- Reliable methods for isolating bovine peripheral blood DCs are lacking, hindering research into their phenotypes and functions.
Purpose of the Study:
- To identify and characterize bovine peripheral blood dendritic cells (DCs).
- To establish a method for isolating bovine DCs from peripheral blood.
Main Methods:
- Negative-selection technique applied to bovine peripheral blood mononuclear cells (PBMC).
- Phenotypic characterization of identified cell populations using specific markers.
Main Results:
- A novel DC phenotype in bovine peripheral blood was identified as CD11c+/CD172a+.
- These CD11c+/CD172a+ cells exhibit characteristics of myeloid-type DCs.
- CD11c+/CD172a+ and CD1+/CD172a+ DC populations were also detected in the thymic medulla.
Conclusions:
- Bovine peripheral blood DCs (CD11c+/CD172a+) are likely derived from myeloid lineages and may lack CD1 expression initially.
- These cells migrate to tissues and may express CD1 upon functional differentiation, suggesting a developmental pathway for bovine DCs.
Abstract:
Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation.
