Related Experiment Video

Updated: Aug 8, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Platelet-derived growth factor BB-mediated normalization of dermal interstitial fluid pressure after mast cell

Asa Lidén1, Ansgar Berg, Torbjørn Nedrebø

  • 1Department of Medical Biochemistry and Microbiology, University of Uppsala, Uppsala, Sweden.

Circulation Research
|February 4, 2006
PubMed

Insights

Platelet-derived growth factor (PDGF)-BB normalizes interstitial fluid pressure during inflammation. This involves alphaVbeta3 integrin, which mediates tensile forces in connective tissue cells.

Area of Science:

  • Integrin biology
  • Connective tissue physiology
  • Inflammatory response

Background:

  • Interstitial fluid pressure (PIF) is crucial for regulating fluid balance in tissues.
  • Cell-mediated control of PIF is essential for maintaining normal tissue fluid content.
  • Beta1 integrins contribute to dermal PIF by mediating cellular tensile strength on the extracellular matrix.

Purpose of the Study:

  • To investigate the role of integrins in regulating interstitial fluid pressure during inflammatory responses.
  • To elucidate the mechanism by which platelet-derived growth factor-BB (PDGF-BB) normalizes PIF.
  • To identify the specific integrins involved in PDGF-BB-mediated PIF regulation.

Main Methods:

  • Utilized mouse models, including beta3-integrin-negative mice.
  • Administered anti-integrin antibodies (anti-beta1 and anti-beta3) and cyclic RGD peptides.
  • Assessed PIF changes following mast cell degranulation and anaphylaxis.
  • Evaluated collagen gel contraction by fibroblasts with varying integrin expression.

Main Results:

  • PDGF-BB normalized PIF reduced by anaphylaxis.
  • Anti-beta3 integrin antibodies and RGD peptides blocked PDGF-BB's ability to normalize PIF.
  • PDGF-BB failed to normalize PIF in beta3-negative mice.
  • Anti-beta1 integrin antibodies reduced PIF in normal dermis but did not affect PDGF-BB's normalization effect.
  • Collagen gel contraction was dependent on alphaVbeta3 integrin, particularly in the presence of PDGF-BB.

Conclusions:

  • AlphaVbeta3 integrin plays a critical role in controlling PIF during inflammatory reactions.
  • PDGF-BB stimulates connective tissue cells to generate tensile forces through alphaVbeta3 integrin during inflammation.
  • This study reveals a novel in vivo function for alphaVbeta3 integrin in regulating fluid dynamics during inflammation.

More Related Videos

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

Related Concept Videos

Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...