Uterine leucocyte infiltration after artificial insemination in bitches

Area of Science:

• Veterinary Medicine
• Reproductive Biology
• Immunology

Background:

• Artificial insemination (AI) in bitches is a common reproductive technique.
• Uterine inflammation can negatively impact reproductive success.
• The role of seminal plasma and extenders in post-AI uterine inflammation requires further investigation.

Purpose of the Study:

• To quantify polymorphonuclear neutrophils (PMN) as indicators of acute uterine inflammation in bitches post-AI.
• To assess the immunomodulating potential of canine seminal plasma.
• To determine if commonly used semen extenders contribute to uterine inflammation.

Main Methods:

• Enumeration of PMN in uterine samples from bitches after AI.
• Analysis of canine seminal plasma for immunomodulatory properties.
• Evaluation of Tris glucose with egg yolk and glycerol extender for inflammatory potential.

Main Results:

• Canine seminal plasma demonstrated an immunomodulating action.
• The Tris glucose with egg yolk and glycerol extender was identified as a potential inducer of uterine inflammation.
• PMN levels indicated acute uterine inflammation following AI.

Conclusions:

• Canine seminal plasma possesses inherent immunomodulatory capabilities.
• Standard semen extenders, particularly Tris glucose with egg yolk and glycerol, may provoke uterine inflammation in bitches.
• Careful consideration of semen extenders is crucial to minimize post-AI uterine inflammation and optimize canine reproduction.