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Muscarinic receptor-activated cationic channels in murine ileal myocytes

A V Dresviannikov1, T B Bolton, A V Zholos

  • 1Department of Nerve-Muscle Physiology, Laboratory of Molecular Pharmacology of Cellular Receptors and Ion Channels, Bogomoletz Institute of Physiology, Kiev, Ukraine.

British Journal of Pharmacology
|August 9, 2006
PubMed

Insights

Researchers investigated excitatory cholinergic mechanisms in mouse small intestine using patch-clamp techniques. They identified key cation channels and their properties, crucial for understanding muscarinic receptor function in this model.

Area of Science:

  • Physiology
  • Molecular Biology
  • Pharmacology

Background:

  • Limited information exists on excitatory cholinergic mechanisms in the mouse small intestine.
  • The mouse small intestine is a vital model for gene knockout studies.

Purpose of the Study:

  • To investigate the voltage-dependent and pharmacological properties of cationic channels in mouse ileal myocytes.
  • To elucidate the excitatory cholinergic mechanisms in the mouse small intestine.

Main Methods:

  • Patch-clamp techniques were employed to study carbachol- or intracellular GTPgammaS-activated cationic channels.
  • Single-channel and whole-cell currents were analyzed in mouse ileal myocytes.

Main Results:

  • Three types of cation channels (17, 70, and 140 pS) were identified in outside-out patches.
  • The 70 pS channel exhibited voltage-dependent behavior consistent with the whole-cell muscarinic current.
  • Channel conductance and open probability varied with permeant cations (Cs+ > Rb+ > Na+ > Li+), and whole-cell current was inhibited by divalent cations, quinine, SK&F 96365, and La3+.

Conclusions:

  • The muscarinic cation current in murine small intestine shares similarities with guinea-pig myocytes.
  • Murine genetic manipulation studies are expected to provide significant insights into muscarinic receptor transduction mechanisms.
Abstract

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