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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
14:06

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Published on: April 16, 2013

ZAP-70 expression is low in normal precursor B cells or hematogones

Bruce H Davis1, Michael Schwartz

  • 1US Labs, Irvine, CA, USA. bdavis@uslabs.net

Cytometry. Part B, Clinical Cytometry
|August 15, 2006
PubMed

Insights

Zeta-associated protein (ZAP-70) is not expressed in immature B-cells (hematogones). Recognizing hematogones is crucial to avoid misinterpreting ZAP-70 expression in chronic lymphocytic leukemia (CLL) prognostic studies.

Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Zeta-associated protein (ZAP-70) expression correlates with a poorer prognosis in chronic lymphocytic leukemia (CLL).
  • The function of ZAP-70 in immature B-cells remains unclear.
  • Immature B-lymphocytes, or hematogones, are common in clinical samples and express CD10, CD38, and CD19.

Purpose of the Study:

  • To investigate the role of ZAP-70 expression in the spectrum of B-cells found in clinical specimens.
  • To determine if ZAP-70 is expressed in hematogones.

Main Methods:

  • Evaluated ZAP-70 expression in 25 patient samples with significant CD10(+)/CD38(bright)/CD19(+) coexpression.
  • Assessed ZAP-70 levels in CD38(bright)/CD19(+) coexpressing cells using routine clinical testing methods for CLL.
  • Hematogone expression ranged from 2-18% of total leukocytes across various conditions.

Main Results:

  • ZAP-70 was consistently absent in CD5-negative, CD38/CD19 coexpressing precursor B-cells (hematogones).
  • Hematogones did not exhibit significant ZAP-70 expression in any of the cases studied.

Conclusions:

  • The presence of hematogones in clinical samples must be acknowledged.
  • Failure to recognize hematogones could negatively impact prognostic assessments of ZAP-70 and CD38 in CLL patients.
Abstract

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