Related Experiment Video
Updated: Jul 19, 2026
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
Analysis of immune cell infiltrates during squamous carcinoma development
Simon R Junankar1, Alexandra Eichten, Annegret Kramer
1Cancer Research Institute, University of California, San Francisco, San Francisco, California 94143, USA.
Insights
Investigating immune cell infiltration in squamous cell carcinoma requires advanced methods. This study details techniques to identify and quantify diverse leukocytes during neoplastic progression, aiding cancer research.
Area of Science:
- Immunology
- Oncology
- Pathology
Background:
- Leukocyte infiltration is a hallmark of physiological and pathological processes.
- Traditional histopathology limits the appreciation of diverse immune cell populations in diseased tissues.
- Specific leukocytes infiltrating squamous tissues are implicated in squamous carcinoma pathogenesis.
Purpose of the Study:
- To develop and detail methodologies for evaluating immune cell types in neoplastic squamous tissues.
- To quantify changes in leukocyte presence and localization during multi-stage cancer progression.
- To enhance the understanding of immune cell roles in squamous carcinogenesis.
Main Methods:
- Utilized flow cytometry for immune cell phenotyping.
- Employed histochemical detection with lineage-selective markers.
- Applied these methods to analyze immune cell infiltration during neoplastic progression.
Main Results:
- Successfully identified and quantified diverse leukocyte populations within squamous neoplastic tissues.
- Demonstrated changes in immune cell presence and localization correlating with neoplastic progression stages.
- Provided a detailed methodological framework for such analyses.
Conclusions:
- The described methodologies enable precise evaluation of immune cell infiltration in squamous carcinogenesis.
- Understanding immune cell dynamics is crucial for elucidating the pathogenesis of squamous cell carcinomas.
- This work facilitates further research into immune cell contributions to cancer development and progression.
Abstract:
Infiltration of leukocytes into tissue is a common feature of many physiological and pathological conditions. Histopathologically, the diversity of leukocytes that infiltrate a tissue associated with a pathophysiologic response cannot be appreciated and/or examined unless highly selective immunologic detection methods are utilized. Specific populations of infiltrating leukocytes into squamous tissues harboring pre-malignant and/or malignant keratinocytes have recently been demonstrated to play a functionally significant role in the pathogenesis of squamous carcinomas. To evaluate immune cell types and quantify changes in their relative presence and localization during multi-stage neoplastic progression, we performed flow cytometry and histochemical detection using lineage-selective markers. Herein, we provide detailed methodology facilitating these analyses.
More Related Videos
07:49Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
Published on: April 13, 2015
07:43Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
Published on: July 29, 2017