Related Experiment Video

Updated: Aug 8, 2026

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
09:58

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid

Published on: January 31, 2012

Expression of intercellular adhesion molecule 1 (ICAM-1) on human articular cartilage chondrocytes

M E Davies1, J T Dingle, R Pigott

  • 1Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.

Connective Tissue Research
|January 1, 1991
PubMed

Insights

Intercellular adhesion molecule 1 (ICAM-1) is not naturally present on cartilage cells but can be induced by interleukin-1 alpha (IL-1α). This molecule

Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Chondrocytes are key cells in articular cartilage.
  • Intercellular adhesion molecule 1 (ICAM-1) plays a role in cell-cell interactions.

Purpose of the Study:

  • To investigate the expression and induction of ICAM-1 on human articular chondrocytes.
  • To determine the role of interleukin-1 alpha (IL-1α) in ICAM-1 expression.

Main Methods:

  • Monoclonal antibodies were used to detect ICAM-1.
  • Chondrocytes were cultured in vitro with varying concentrations of IL-1α.
  • Time-course experiments were conducted to assess expression kinetics.

Main Results:

  • ICAM-1 is not constitutively expressed on chondrocytes.
  • IL-1α induces ICAM-1 expression in a dose-dependent manner (0.01-20 ng/ml), with optimal expression at 2-5 ng/ml.
  • Induced ICAM-1 expression was observed after 16 hours and sustained for at least 6 days.
  • Endothelial leukocyte adhesion molecule 1 (ELAM-1) was not expressed or induced.

Conclusions:

  • IL-1α is a potent inducer of ICAM-1 on human articular chondrocytes.
  • The sustained expression of ICAM-1 suggests its potential role in cartilage inflammatory processes.
  • Chondrocytes do not express ELAM-1 under these conditions.

More Related Videos

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions07:40

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants08:42

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Published on: January 7, 2019

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Anchoring Junctions01:03

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...