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Updated: Jul 17, 2026
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
Published on: April 21, 2019
A novel piezoelectric quartz micro-array immunosensor for detection of immunoglobulinE
Chunyan Yao1, Qinghai Chen, Ming Chen
1Department of Clinical Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Insights
A novel piezoelectric micro-array immunosensor enables faster, quantitative detection of human immunoglobulin E (IgE) in serum. This multi-channel sensor offers high precision and reusability for IgE assays.
Area of Science:
- Biosensors
- Analytical Chemistry
- Immunosensing
Background:
- Quantitative detection of human immunoglobulin E (IgE) is crucial for diagnosing allergic conditions.
- Traditional immunosensors often face limitations in detection speed and throughput.
- Development of sensitive and rapid diagnostic tools for IgE is an ongoing area of research.
Purpose of the Study:
- To develop and characterize a novel multi-channel piezoelectric (PZ) micro-array immunosensor for the quantitative detection of human IgE in serum.
- To evaluate the performance of the developed immunosensor in terms of detection speed, precision, and accuracy compared to established methods.
- To assess the reusability and stability of the fabricated immunosensor.
Main Methods:
- Fabrication of a 2 x 5 multi-channel piezoelectric micro-array immunosensor using 10 MHz AT-cut quartz crystals.
- Immobilization of anti-IgE antibodies onto gold electrode surfaces via staphylococcal protein A (SPA) to create antibody monolayers.
- Quantitative detection of human IgE in serum samples within a specific concentration range.
- Comparison of results with commercially available ELISA test kits and assessment of reusability after regeneration.
Main Results:
- The multi-channel PZ immunosensor achieved eight times higher detection speeds compared to traditional one-channel sensors.
- The sensor demonstrated high precision (CV of 4%) for human IgE determination in the range of 5-300 IU/ml.
- Excellent agreement was observed between the PZ immunosensor and ELISA, with a correlation coefficient of 0.94.
- The immunosensor could be reused up to 6 times without significant loss of activity after NaOH regeneration.
Conclusions:
- The developed multi-channel piezoelectric micro-array immunosensor is a highly effective tool for rapid and quantitative detection of human IgE in serum.
- The sensor's design ensures non-interfering oscillation of crystal units, leading to improved performance and higher throughput.
- The high precision, accuracy, and reusability of the PZ immunosensor make it a promising alternative to conventional diagnostic methods for IgE quantification.
Abstract:
A novel multi-channel 2 x 5 model of piezoelectric (PZ) micro-array immunosensor has been developed for quantitative detection of human immunoglobulinE (IgE) in serum. Every crystal unit of the fabricated piezoelectric IgE micro-array immunosensor can oscillate without interfering each other. A multi-channel 2 x 5 model micro-array immunosensor as compared with the traditional one-channel immunosensor can provide eight times higher detection speeds for IgE assay. The anti-IgE antibody is deposited on the gold electrode's surface of 10 MHz AT-cut quartz crystals by SPA (staphylococcal protein A), and serves as an antibody recognizing layer. The highly ordered antibody monolayers ensure well-controlled surface structure and offer many advantages to the performance of the sensor. The uniform amount of antibody monolayer coated by the SPA is good, and non-specific reaction caused by other immunoglobulin in sample is found. The fabricated PZ immunosensor can be used for human IgE determination in the range of 5-300 IU/ml with high precision (CV is 4%). 50 human serum samples were detected by the micro-array immunosensor, and the results agreed well with those given by the commercially ELISA test kits. The correlation coefficient is 0.94 between ELISA and PZ immunosensor. After regeneration with NaOH the coated immunosensor can be reused 6 times without appreciable loss of activity.
