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Published on: May 31, 2014
Molecular cloning and characterization of Duck CD25
Jinyong Wang1, Jie Fang, Junqing Guo
1Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310029, China.
Insights
Researchers identified a duck interleukin-2 receptor alpha chain (duCD25) crucial for T-cell responses. duCD25 positive cells increase during avian influenza virus (H9N2) infection, suggesting a role in duck immunity.
Area of Science:
- Immunology
- Avian Virology
Background:
- The interleukin-2 receptor alpha chain (CD25) is vital for T-cell immune responses in mammals.
- No duck cytokine receptors had been previously described, leaving a gap in understanding avian immune signaling.
Purpose of the Study:
- To isolate and characterize a duck homolog of the IL-2Ralpha chain (duCD25).
- To investigate the role of duCD25 in duck immune responses, particularly during avian influenza virus (H9N2) infection.
Main Methods:
- Isolation of the duck cytokine receptor (duCD25) cDNA segment.
- Generation of a novel mouse monoclonal antibody (mAb) against duck CD25.
- Localization of duCD25 on duck lymphocytes using the mAb.
- Inhibition of duck IL-2-induced lymphocyte proliferation.
- Flow cytometry analysis of duCD25 expression in healthy and H9N2-infected ducks.
Main Results:
- A cDNA segment encoding duck CD25 (duCD25) was successfully isolated.
- A specific mAb was developed, confirming duCD25 presence on duck lymphocytes.
- duCD25 engagement inhibited duck IL-2-mediated lymphocyte proliferation.
- duCD25 positive cells were significantly upregulated in ducks infected with avian influenza virus (H9N2).
Conclusions:
- duCD25 functions as the receptor for duck interleukin-2.
- duCD25 positive cells are implicated in the duck immune response to H9N2 viral infection.
Abstract:
The IL-2Ralpha chain (CD25, Tac) is an essential component of high affinity IL-2Rs, playing critical role for the immune specificity of antigen-activated T-cell clonal expansion. Up to now, no duck cytokine receptor has been described. Here, the cDNA segment of a duck cytokine receptor (duCD25), encoding a 226 aa precursor protein with a 20 aa signal peptide, was isolated. Then a novel mouse monoclonal antibody (mAb) was generated using the prokaryotically expressed duCD25 protein as immunogen. Using this mAb, the endogenous duCD25 molecule was localized on the surface of duck lymphocytes, and the duck IL-2-induced lymphocyte proliferation was further inhibited. Furthermore, flow cytometry analysis showed that duCD25 positive cells were upregulated in ducks infected with avian influenza virus (H9N2). Our findings confirm that duCD25 is a receptor of duck interleukin-2, and duCD25 positive cells play a potential role in H9N2 virus infection.
