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Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
11:58

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching

Published on: February 29, 2012

[In the dying cell secretory protein diffuses through the membranes into the cytosol]

V G Sipovskiĭ, R I Valeev, A I Nevorotin

    Arkhiv Patologii
    |January 1, 1991
    PubMed

    Insights

    Post-mortem changes in hybridoma lymphoblasts reveal that anti-horseradish peroxidase IgG (a-HRP) initially confined to cellular organelles begins to diffuse into the cytosol. This diffusion, observed 30-120 minutes after death, suggests significant post-mortem alterations in intracellular membrane permeability.

    Area of Science:

    • Cell Biology
    • Immunology
    • Histology

    Context:

    • Study investigates the intracellular localization and post-mortem behavior of anti-horseradish peroxidase IgG (a-HRP) in hybridoma lymphoblasts.
    • Utilizes light and electron microscopy with conventional HRP-DAB immunocytochemical staining.

    Purpose:

    • To examine the distribution of a-HRP within living hybridoma lymphoblasts.
    • To observe changes in a-HRP localization following animal death and assess post-mortem membrane permeability.

    Summary:

    • In living cells, a-HRP was localized to the rough endoplasmic reticulum, perinuclear cisterns, Golgi apparatus, and microvesicles.
    • 30 minutes post-mortem, a-HRP started invading the cytosol, sparing the nucleus and mitochondrial matrix.
    • By 90 minutes post-mortem, staining intensity was uniform across cellular structures, indicating widespread diffusion and compromised membrane integrity.

    Impact:

    • Findings suggest that macromolecules can diffuse across intracellular membranes due to significant post-mortem disturbances in membrane permeability.
    • Provides insights into cellular degradation processes and the reliability of immunocytochemical markers in post-mortem studies.

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