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Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Activation of human monocytes by interleukin 2: role of T lymphocytes

J Ennen1, M Ernst, H D Flad

  • 1Forschungsinstitut Borstel, Department of Immunology and Cell Biology, FRG.

Journal of Bioluminescence and Chemiluminescence
|January 1, 1991
PubMed

Insights

Interleukin 2 (IL2) primes human monocytes for enhanced reactive oxygen species secretion via Fc-gamma receptors, but requires T-cell interaction. This IL2-driven monocyte and T-cell cooperation boosts Fc-gamma R-mediated activity.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Interleukin 2 (IL2) is a key cytokine in immune regulation.
  • Monocytes play a crucial role in innate immunity, including the respiratory burst.
  • Fc-gamma receptors (Fc-gamma R) on monocytes mediate phagocytosis and inflammatory responses.

Purpose of the Study:

  • To investigate the effect of IL2 on human monocyte reactive oxygen species (ROS) secretion.
  • To determine the role of T cells in IL2-mediated monocyte activation.
  • To elucidate the mechanisms underlying IL2-induced monocyte priming and activation.

Main Methods:

  • Chemiluminescence (CL) assays were used to measure ROS production.
  • Highly purified (hp) human monocytes were used, with and without co-culture of T cells.
  • Monoclonal antibodies were employed to block specific cytokine and receptor interactions.

Main Results:

  • IL2 alone did not directly enhance ROS production in hp monocytes.
  • Co-culture of hp monocytes with T cells was necessary for IL2 to enhance Fc-gamma R-mediated ROS secretion.
  • IL2 pretreatment primed monocytes, leading to enhanced Fc-gamma R-mediated CL response upon subsequent T-cell co-culture.
  • IL2-induced monocyte activation and MAF secretion were dependent on IL2 receptor interaction.

Conclusions:

  • IL2-driven monocyte-T cell interaction enhances Fc-gamma R-mediated monocytic respiratory burst activity.
  • IL2 primes monocytes for enhanced responses, dependent on IL2 receptor signaling.
  • Soluble monocyte activating factor (MAF) activity is secreted, but interferon-gamma (IFN-gamma) was not detected in IL2-stimulated T-cell dependent monocyte cultures.

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