Enumeration of absolute cell counts using immunophenotypic techniques

Area of Science:

• Clinical immunology
• Hematology
• Biotechnology

Background:

• Accurate cell enumeration is vital for managing HIV/AIDS, immune disorders, and ensuring blood product safety.
• Traditional methods face challenges in precision and efficiency for absolute cell counts.

Purpose of the Study:

• To present detailed protocols for absolute cell counting using flow cytometry.
• To highlight the single-platform method as the preferred approach for cell enumeration.

Main Methods:

• Utilizes flow cytometry as a single platform for absolute cell enumeration.
• Employs electronic gating algorithms with lineage-specific immunofluorescent markers for precise cell identification.
• Describes protocols for both volumetric and flow-rate determination of residual white blood cells and leukocyte subsets.

Main Results:

• The single-platform flow cytometry method enables precise and automatic exclusion of unwanted cells.
• Provides validated protocols for accurate enumeration of residual white blood cells and leukocyte subsets.

Conclusions:

• Single-platform flow cytometry is the method of choice for absolute cell enumeration in clinical settings.
• The presented protocols facilitate reliable monitoring of disease status and immune function.