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Updated: Jul 1, 2026

Immunoblot Analysis
Published on: June 20, 2008
[Analytical methods at the boundary between biochemistry and immunology]
1Ustav klinické biochemie a laboratorní diagnostiky 1. LF UK a VFN, Praha. petr.stern@vfn.cz
Insights
This guide details over 60 shared laboratory tests for Clinical Biochemistry and Laboratory of Allergology and Immunology, focusing on pre-analytical factors crucial for general practitioners and lab professionals.
Area of Science:
- Clinical Biochemistry
- Laboratory of Allergology and Immunology
Context:
- Provides reference information for general practitioners and novice laboratory workers.
- Highlights the importance of the pre-analytical stage in laboratory testing.
Purpose:
- To offer a comprehensive overview of over 60 shared assays between Clinical Biochemistry and Laboratory of Allergology and Immunology.
- To guide general practitioners on essential aspects of ordering and interpreting laboratory determinations.
Summary:
- Covers selected analyses including proteins, enzymes, tumor markers, ions, osmolality, and quantitative microscopic urinalysis.
- Emphasizes pre-analytical factors like specimen collection, transport, storage, analytical reliability, accessibility, frequency, and costs.
- Analytical techniques are mentioned marginally, focusing on practical application for general practitioners.
Impact:
- Aims to improve the understanding and application of laboratory diagnostics for healthcare professionals.
- Facilitates better utilization of laboratory services by general practitioners.
- Serves as a foundational resource for laboratory workers entering the fields of clinical biochemistry and allergology/immunology.
Abstract:
The article presents assays shared by both disciplines; i.e the specialization 801 (Clinical Biochemistry) and 813 (Laboratory of Allergology and Immunology), and it covers over 60 tests. It is intended as reference information for general practitioners, as well as for laboratory workers at the beginning of their studies. Emphasis is laid on some facts which GPs ordering laboratory determinations should be familiar with; the pre-analytical stage (specimen collection, their transport and storage), the analytical reliability of particular methods, their accessibility, frequency and total costs. Analytical techniques are only mentioned marginally as their understanding is not essential for GPs. Assuming the readers' fundamental experience of diagnostic applications of the methods discussed; we only comment some obsolete or rare tests. The shared assays cover selected analyses of proteins (incl. some enzymes and tumormarkers), ions, osmolality and quantitative microscopic urinalysis.
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