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Immunocytochemical localization of casein kinase II during interphase and mitosis

I J Yu1, D L Spector, Y S Bae

  • 1Cold Spring Harbor Laboratory, New York 11724.

The Journal of Cell Biology
|September 1, 1991
PubMed

Insights

Casein kinase II (CKII) subunits show dynamic localization. CKII alpha and beta are cytoplasmic in interphase, throughout the cell in mitosis, and alpha associates with spindle fibers, suggesting roles in cell division.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Casein kinase II (CKII) is a crucial enzyme involved in various cellular processes.
  • Understanding the spatiotemporal localization of CKII subunits is essential for elucidating its functions.

Purpose of the Study:

  • To investigate the subcellular localization of casein kinase II (CKII) alpha, beta, and alpha' subunits in HeLa cells.
  • To determine how CKII localization changes during the cell cycle, particularly at the G1/S transition and mitosis.

Main Methods:

  • Development of specific antibodies against synthetic peptide antigens for individual CKII subunits.
  • Immunofluorescence and immunoelectron microscopy to visualize CKII subunit localization in asynchronous and hydroxyurea-arrested HeLa cells.

Main Results:

  • CKII alpha and beta subunits are localized in the cytoplasm during interphase and distribute throughout the cell during mitosis.
  • CKII alpha subunit associates with spindle fibers during metaphase and anaphase.
  • CKII alpha' subunit exhibits nuclear localization during G1 and cytoplasmic localization during S phase.

Conclusions:

  • CKII subunits display dynamic subcellular localization patterns throughout the cell cycle.
  • The observed shifts in CKII localization suggest a significant role in regulating cell division and cell cycle progression.

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