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Published on: March 26, 2018
[Analysis of immunophenotypes of acute lymphoblastic leukemia by three color flow cytometry]
Yang Qiu1, Gang Wang, Yue-Jian Liu
1The First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
Insights
This study accurately identified acute lymphoblastic leukemia (ALL) subtypes using flow cytometry. CD45/SSC gating enhances immunophenotype analysis for reliable ALL subtyping.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- Acute lymphoblastic leukemia (ALL) is a heterogeneous hematologic malignancy.
- Accurate immunophenotyping is crucial for classifying ALL subtypes and guiding treatment.
- Traditional methods can be confounded by normal hematopoietic cells.
Purpose:
- To investigate immunophenotype characteristics in ALL subtypes.
- To evaluate the efficacy of three-color flow cytometry with CD45/SSC gating for ALL analysis.
- To determine the diagnostic significance of specific immunophenotypic markers in ALL.
Summary:
- Forty ALL cases were analyzed using three-color flow cytometry with CD45/SSC two-parametric gating.
- This method reliably distinguished ALL cells from normal hematopoietic populations.
- B-ALL (26 cases) showed 100% CD19 expression, T-ALL (11 cases) showed 100% CD17 expression.
- Myeloid antigen-positive ALL (My-ALL) occurred in 30% of cases, with CD13 being the most common myeloid antigen.
- Hybrid ALL (HAL) cases (3) coexpressed myeloid and B-lineage antigens, with CD34 in 66.67%.
Impact:
- Three-color flow cytometry with CD45/SSC gating provides a reliable and accurate method for ALL immunophenotyping.
- This technique minimizes interference from normal cells, improving diagnostic accuracy.
- Enhanced subtyping facilitates personalized treatment strategies for ALL patients.
Abstract:
This study was purposed to investigate the immunophenotype characteristics and their significance in subtypes of acute lymphoblastic leukemia (ALL). The immunophenotypes in 40 cases of ALL were analyzed with three color flow cytometry using CD45/SSC two-parametric gating. The results showed that the three color flow cytometry assay using CD45/SSC two-parametric gating could accurately distinguish each other from lymphocytes, monocytes, granulocytes, erythroblasts and primitive cells in bone marrow and/or peripheral blood. Among 40 cases of ALL, B-ALL was 26 cases, T-ALL was 11 cases, HAL was 3 cases. All of the 26 cases of B-ALL expressed CD19 with positive rate of 100%, meanwhile 11 cases of T-ALL most highly expressed CD17 with positive rate of 100%. 12 cases of ALL with myeloid antigen expression (My-ALL) were involved in ALL, the incidence of these cases was almost 30% (12/40). The CD13 was expressed most highly in myeloid antigens. All 3 cases of HAL coexpressed myeloid and B-lineage antigens, among them CD34 was expressed in 2 cases with positive rate of 66.67%. It is concluded that three color flow cytometry assay using CD45/SSC two-parametric gating can exclude the interference of normal cells, thereby the results are more reliable and more accurate.
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