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Updated: Jun 20, 2026
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
[Screening study methods use for substantiation of immune modulating therapy of hypergic inflammatory process in
Insights
Patients with maxillofacial inflammation show reduced lymphocytes. Lower lymphocyte counts correlate with decreased CD3 and CD4 cells, indicating secondary immune deficiency.
Area of Science:
- Immunology
- Maxillofacial Surgery
- Flow Cytometry
Background:
- Hypergic inflammatory processes in the maxillofacial region can impact systemic immunity.
- Understanding immune cell dynamics is crucial for managing complex inflammatory conditions.
Purpose of the Study:
- To investigate lymphocyte count alterations in patients with maxillofacial inflammatory conditions.
- To determine the correlation between peripheral blood lymphocyte numbers and specific T-cell subpopulations (CD3, CD4).
Main Methods:
- Flow cytometry was employed to analyze lymphocyte populations.
- Correlation analysis was used to assess relationships between absolute lymphocyte counts and CD3/CD4 subpopulations.
Main Results:
- Patients exhibited a reduction in both relative and absolute lymphocyte numbers.
- A strong, direct correlation was observed between absolute lymphocyte count and absolute CD3 and CD4 counts.
Conclusions:
- Reduced peripheral blood lymphocyte counts likely reflect diminished CD3 and CD4 subpopulations.
- These findings suggest a potential for diagnosing secondary immune deficiency in affected patients.
Abstract:
With the help of flow cytometry it was shown that for patients with hypergic inflammatory process in maxillofacial region the reduction of lymphocytes relative number was typical as well as their absolute number reduction. Direct and very strong correlation was established between absolute lymphocytes number in peripheral blood and absolute number of CD3 and CD4 lymphocytes subpopulations by correlation analyses. The reduction of absolute lymphocytes number in peripheral blood let speak with more probability about the reduction of the number of CD3 and CD4 lymphocytes subpopulations and diagnose secondary immune deficit.
