Related Experiment Video
Updated: Aug 9, 2026
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
Published on: October 1, 2007
Immunohistochemical determination of CD23 expression in Hodgkin's disease using paraffin sections
D C Rowlands1, T T Hansel, J Crocker
1Department of Histopathology, East Birmingham Hospital, U.K.
Insights
CD23, a leukocyte antigen, is expressed in Hodgkin's disease, B-cell, and T-cell lymphomas. This finding suggests CD23's potential role in the characteristic cellular infiltrate of Hodgkin's lymphoma.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Leukocyte antigen CD23 plays roles in B-cell development, acting as an IgE receptor and lymphocyte growth factor.
- Understanding CD23 expression is crucial for diagnosing and understanding lymphoid malignancies.
Purpose of the Study:
- To investigate the expression patterns of CD23 in various lymphoid tissues and tumors.
- To determine the potential involvement of CD23 in the pathogenesis of Hodgkin's lymphoma.
Main Methods:
- Immunohistological analysis of paraffin-embedded lymphoid tissue sections.
- Utilized the monoclonal antibody BU38 for CD23 detection.
- Analyzed cases of Hodgkin's disease, reactive lymph nodes, and non-Hodgkin's lymphomas (B-cell and T-cell).
Main Results:
- CD23 was detected on follicular dendritic cells and some lymphocytes in reactive lymph nodes.
- Thirteen out of 15 Hodgkin's disease cases exhibited CD23 expression in neoplastic cells and the surrounding infiltrate.
- CD23 was also found in a subset of B-cell (4/8) and T-cell (1/7) non-Hodgkin's lymphomas.
Conclusions:
- CD23 expression is a notable feature in Hodgkin's disease, extending beyond normal B-cell populations.
- The presence of CD23 in both neoplastic and reactive cells suggests its potential contribution to the characteristic inflammatory microenvironment of Hodgkin's lymphoma.
- CD23 warrants further investigation for its role in lymphoid neoplasms, particularly Hodgkin's lymphoma.
Abstract:
The leukocyte antigen CD23 is expressed during B-cell development, and functions as an IgE receptor and a lymphocyte growth factor. We studied the expression of CD23 in paraffin sections of lymphoid tissue using the monoclonal antibody BU38. Fifteen cases of Hodgkin's disease, ten reactive lymph nodes, eight B-cell, and seven T-cell non-Hodgkin's lymphomas were analysed immunohistologically. CD23 positivity was seen on follicular dendritic cells and a small number of lymphocytes in reactive nodes. Thirteen of the 15 cases of Hodgkin's disease showed CD23 expression in both neoplastic cells and reactive lymphocytic infiltrate. The antigen was demonstrated in four of the B-cell and one of the T-cell tumours. CD23 may be important in mediating the mixed cellular infiltrate characteristic of Hodgkin's lymphoma.
