Related Experiment Video
Updated: Jun 1, 2026
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
A novel liquid-phase piezoelectric immunosensor for detecting Schistosoma japonicum circulating antigen
Zhili Wen1, Shiping Wang, Zhaoyang Wu
1Infectious Disease Department, Nanchang NO.1 Hospital ( NO.3 affiliated hospital of Nanchang University), Nanchang, China. wenzhili@126.com
Insights
A novel liquid-phase piezoelectric immunosensor (LP-PEIS) was developed to quantify Schistosoma japonicum (Sj) circulating antigens (SjCAg). This new method shows promise for detecting Sj infection with high accuracy.
Area of Science:
- Immunoscience
- Biomedical Engineering
- Parasitology
Background:
- Schistosomiasis japonica remains a significant public health concern.
- Accurate detection of Schistosoma japonicum circulating antigens (SjCAg) is crucial for diagnosis and monitoring.
- Existing diagnostic methods have limitations in sensitivity and specificity.
Purpose of the Study:
- To develop and validate a new liquid-phase piezoelectric immunosensor (LP-PEIS) for quantitative detection of SjCAg.
- To optimize the LP-PEIS for SjCAg detection.
- To compare the performance of LP-PEIS with the established sandwich ELISA method.
Main Methods:
- Purification of IgG antibodies against Sj from rabbit sera.
- Immobilization of antibodies onto piezoelectric quartz crystals using staphylococcal protein A (SPA).
- Optimization of LP-PEIS conditions using rabbit sera and subsequent testing with human patient sera.
Main Results:
- Optimal conditions for LP-PEIS detection of SjCAg were established.
- LP-PEIS demonstrated a higher positive rate (100%) and lower false positive rate (3.0%) compared to sandwich ELISA (92.8%, 6.0%) in acute Schistosomiasis patients.
- No significant difference was observed between LP-PEIS and sandwich ELISA in overall performance.
Conclusions:
- The developed LP-PEIS is capable of quantitative detection of SjCAg in patient sera.
- LP-PEIS offers comparable diagnostic performance to sandwich ELISA for Schistosoma japonicum detection.
- LP-PEIS presents a viable alternative for the diagnosis of Schistosomiasis japonica.
Aims:
A new liquid-phase piezoelectric immunosensor (LP-PEIS), which can detect Schistosoma japonicum (Sj) circulating antigens (SjCAg) quantificationally, was developed.
Methods:
The IgG antibodies were purified from the sera of rabbits which had been infected or immunized by Sj and were immobilized on the surface of piezoelectric quartz crystal in LP-PEIS by staphylococcal protein A (SPA). It was used to detect SjCAg in sera of rabbits which had been infected by Sj in order to acquire some optimum conditions for detecting SjCAg. Finally, the LP-PEIS with optimum conditions was used to detect SjCAg in sera of patients who had been infected by Sj, and was compared with sandwich ELISA.
Results:
A lot of optimum conditions of LP-PEIS for detecting SjCAg had been acquired. In the detection of patients' sera with acute Schistosomiasis, LP-PEIS has higher positive rate (100%) and lower false positive rate (3.0%) than sandwich ELISA (92.8%, 6.0%). However, there were no significant difference between LP-PEIS and sandwich ELISA.
Conclusions:
LP-PEIS can quantificationally detect SjCAg in patients' sera as well as sandwich ELISA.
