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JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue
D V Parums1, J L Cordell, K Micklem
1University of Oxford, Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Headington.
Insights
A new antibody, JC70, identifies a specific glycoprotein on endothelial cells. This antibody is valuable for diagnosing benign and malignant vascular disorders in routine tissue samples.
Area of Science:
- Immunology
- Pathology
- Oncology
Background:
- Hairy cell leukaemia research led to the development of monoclonal antibody JC70.
- JC70 recognizes a membrane-bound glycoprotein, similar to CD31 antibodies.
- This glycoprotein is present on endothelial cells in various tissues.
Purpose of the Study:
- To evaluate the utility of monoclonal antibody JC70.
- To assess JC70's effectiveness in identifying endothelial cells in benign and malignant conditions.
- To compare JC70 with Factor VIII related antigen (FVIII-Rag) in diagnosing vascular tumors.
Main Methods:
- JC70 was raised against a spleen membrane preparation from a hairy cell leukaemia patient.
- Immunohistochemistry was performed on paraffin-wax embedded tissues.
- JC70 staining was compared to FVIII-Rag staining in angiosarcomas and Kaposi's sarcoma.
Main Results:
- JC70 stains a fixation-resistant epitope on endothelial cells.
- JC70 demonstrated consistent staining of malignant endothelial cells in 10 angiosarcomas.
- JC70 stained malignant endothelial cells, but not spindle cells, in four Kaposi's sarcoma cases.
Conclusions:
- Monoclonal antibody JC70 is valuable for studying human vascular disorders.
- JC70 is effective in routinely processed tissues, offering advantages over FVIII-Rag.
- JC70 aids in distinguishing malignant endothelial cells in vascular tumors.
Abstract:
A new monoclonal antibody, JC70, raised against a membrane preparation from a spleen affected by hairy cell leukaemia, recognises a membrane bound glycoprotein identical with that of the CD31 group of monoclonal antibodies. The antibody stains a fixation resistant epitope on endothelial cells in benign and malignant conditions in a wide variety of paraffin wax embedded tissue. JC70 stained malignant endothelial cells in 10 angiosarcomas with more consistency than monoclonal or polyclonal antibodies to factor VIII related antigen (FVIII-Rag). In four cases of Kaposi's sarcoma the antibody stained malignant endothelial cells but not spindle cells. It is concluded that antibody JC70 is of value for studying benign and malignant human vascular disorders in routinely processed tissue.
