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Functional immunoimaging: the revolution continues

Philippe Bousso1, Hélène D Moreau

  • 1Institut Pasteur, Dynamics of Immune Responses Unit, F-75015 Paris, France. philippe.bousso@pasteur.fr

Nature Reviews. Immunology
|November 24, 2012
PubMed

Insights

Dynamic in vivo imaging, particularly two-photon imaging, revolutionized the study of immune responses. Recent advances now allow simultaneous investigation of immune cell dynamics and function, enhancing our understanding of in situ immune cell communication.

Area of Science:

  • Immunology and biomedical imaging

Background:

  • Dynamic in vivo imaging, introduced in 2002, transformed the study of immune responses.
  • Two-photon imaging has been pivotal in understanding immune cell dynamics across diverse conditions like infection, cancer, and autoimmunity.

Purpose of the Study:

  • To highlight the evolution of intravital imaging in immunology.
  • To showcase recent advancements enabling simultaneous analysis of immune cell dynamics and function.
  • To underscore the expanded potential of immunoimaging for in situ immune cell communication research.

Main Methods:

  • Utilizing dynamic in vivo imaging techniques, specifically two-photon imaging.
  • Analyzing immune cell migration and interactions within living organisms.
  • Investigating coupled cell dynamics and function through advanced intravital imaging.

Main Results:

  • Initial studies focused on immune cell migration and interactions.
  • Recent advancements enable simultaneous assessment of immune cell dynamics and function.
  • Immunoimaging applications have significantly broadened.

Conclusions:

  • Dynamic in vivo imaging has become a cornerstone in immunological research.
  • Simultaneous investigation of immune cell dynamics and function represents a significant leap forward.
  • Intravital imaging offers unprecedented opportunities to decode complex immune cell communication in situ.

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