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Updated: May 15, 2026
Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
Flow cytometry for non-Hodgkin and classical Hodgkin lymphoma
David Wu1, Brent L Wood, Jonathan R Fromm
1Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
Insights
Multiparametric flow cytometry aids in diagnosing lymphoid disorders by identifying abnormal B, T, and Hodgkin lymphoma cells. Understanding normal cell antigen expression is key for accurate disease classification and monitoring.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- Multiparametric flow cytometry (MFC) is a crucial diagnostic technique for rapid cellular antigen assessment.
- Immunophenotypic information from MFC aids in disease classification, particularly for lymphoid malignancies.
- Understanding normal lymphoid cell antigen expression is vital for identifying abnormal populations.
Purpose of the Study:
- To outline a general approach for identifying abnormal lymphoid populations using MFC.
- To provide strategies for the diagnosis of non-Hodgkin B- and T-cell lymphomas.
- To detail a method for the sensitive and specific diagnosis of classical Hodgkin lymphoma.
Main Methods:
- General approach for identifying abnormal lymphoid populations via MFC.
- Overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas.
- Specific strategy for diagnosing classical Hodgkin lymphoma.
Main Results:
- MFC enables rapid assessment of cellular antigen expression for disease classification.
- Identification of abnormal B, T, and Hodgkin lymphoma cells is achievable.
- The described methods support clinical and research environments.
Conclusions:
- MFC is a powerful tool for the immunophenotypic diagnosis of lymphoid diseases.
- Knowledge of normal cell antigen patterns is critical for accurate diagnosis and minimal residual disease assessment.
- The presented strategies enhance the diagnosis of various lymphomas.
Abstract:
Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow cytometry, including B, T, and Hodgkin lymphoma cells suitable for the clinical and research environment. Knowledge of the common patterns of antigen expression of normal lymphoid cells is critical to permit identification of abnormal populations at disease presentation and for minimal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma.
