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Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
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Forming a complex with MHC class I molecules interferes with mouse CD1d functional expression

Renukaradhya J Gourapura1, Masood A Khan, Richard M Gallo

  • 1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.

Plos One
|September 7, 2013
PubMed

Insights

Major Histocompatibility Complex class I (MHC class I) molecules regulate CD1d function by physically associating with CD1d. This association masks CD1d, impacting lipid antigen presentation to Natural Killer T (NKT) cells.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD1d molecules are structurally similar to MHC class I but present lipid antigens.
  • MHC class I molecules typically present peptide antigens.

Purpose of the Study:

  • To investigate the physical association between MHC class I and CD1d molecules.
  • To determine the functional consequences of this association on CD1d-mediated antigen presentation to NKT cells.

Main Methods:

  • Acid stripping of MHC class I molecules from antigen-presenting cells.
  • Analysis of surface expression of murine CD1d.
  • Assessment of CD1d-mediated antigen presentation to NKT cells.
  • Comparison of NKT cell populations and CD1d antigen presentation in TAP1-/- mice versus wild-type mice.

Main Results:

  • MHC class I molecules physically associate with and regulate the surface expression of mouse CD1d.
  • Low pH acid stripping of MHC class I increased surface CD1d expression and augmented CD1d-mediated antigen presentation to NKT cells.
  • TAP1-/- mice exhibited a higher percentage of type I NKT cells and enhanced CD1d antigen presentation by dendritic cells.

Conclusions:

  • MHC class I molecules regulate NKT cell function by masking CD1d.
  • This interaction influences the availability of CD1d for lipid antigen presentation.

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