Related Experiment Video
Updated: May 8, 2026
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
Published on: June 11, 2019
[Morpho-functional characteristics of human spleen (an immunohistochemical study)]
V B Zaitsev1, N S Fedorovskaya, D A Diakonov
1State Medical Academy, Kirov, Russia
Insights
This study quantifies immunocompetent cells in the human spleen, detailing T and B lymphocytes, macrophages, and dendritic cells. Understanding their distribution aids in improving histopathological diagnoses.
Area of Science:
- Immunology
- Histology
- Cell Biology
Context:
- The human spleen plays a crucial role in immune surveillance and hematopoiesis.
- Understanding the precise localization and interplay of immune cells within splenic compartments is vital for immunological research.
Purpose:
- To quantitatively analyze the composition and structural localization of various immunocompetent cell subpopulations in the human spleen.
- To provide detailed morpho-functional characteristics of the splenic white and red pulp.
Summary:
- Immunohistochemical and morphometric techniques were employed on human spleen autopsy samples (n=20).
- The study identified and mapped T lymphocytes (CD3+, CD4+, CD8+), B lymphocytes (CD20+), natural killer cells (CD57+), tissue macrophages (CD68+), and dendritic cells (CD35+, S100+).
- Correlations between these cells in different splenic regions were analyzed.
Impact:
- The findings offer a basis for optimizing histopathological diagnostic criteria in clinical practice.
- This research enhances our understanding of splenic immune cell architecture and function.
Abstract:
Using immunohistochemical and morphometric methods, the quantitative composition and structural localization of immunocompetent cells were studied in human spleen (n=20, autopsy material). The detailed morpho-functional characteristics of splenic white and red pulp are presented. Cell subpopulations of T (CD3+, CD4+, CD8+) and B lymphocytes (CD20+), natural killer cells (CD57+), tissue macrophages (CD68+) and dendritic cells (CD35+, S100+) were studied. An analysis of the correlations of immunocompetent cells in different regions of white and red pulp may become the basis for optimization of histopathological diagnosis definition in clinical practice
