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Updated: Jul 16, 2026
Isolation of Valvular Endothelial Cells
Published on: December 29, 2010
The glycoprofile patterns of endothelial cells in usual interstitial pneumonia
A Barkhordari, C Jp Jones, R W Stoddart
1Occupational Health Department, Neyshabur University of Medical Sciences, Neyshabur, Iran. s.rahimimoghadam@gmail.com.
Insights
This study reveals distinct glycosylation patterns in capillary endothelial cells of usual interstitial pneumonia (UIP), suggesting two unique cell populations involved in lung fibrosis and angiogenesis.
Area of Science:
- Pulmonary pathology
- Glycobiology
- Cellular differentiation
Background:
- Cryptogenic fibrosing alveolitis classification remains controversial.
- Usual interstitial pneumonia (UIP) is a key form of idiopathic pulmonary fibrosis.
Purpose of the Study:
- To characterize the glycotype of capillary endothelial cells in UIP.
- To compare these glycotypes with those in normal lung tissue.
Main Methods:
- Lectin histochemistry was performed on 16 UIP tissue samples.
- A panel of 27 biotinylated lectins and avidin-peroxidase system were utilized.
Main Results:
- UIP endothelial cells showed de novo expression of specific N-linked glycan sequences (Concanavalin A, erythro-phytohemagglutinin).
- Distinct glycan residues (Helix pomatia, Maclura pomifera, Ulex europaeus agglutinin-I) were identified in UIP.
- Absence of certain L-fucosylated type I glycan chains (Anguilla anguilla agglutinin) was noted in UIP compared to normal tissue.
Conclusions:
- UIP may feature two distinct endothelial cell populations with unique glycosylation profiles.
- These differences may reflect underlying patterns of differentiation and angiogenesis in UIP.
- These cellular distinctions are not apparent through standard morphological examination.
Background:
The pathological classification of cryptogenic fibrosing alveolitis has been a matter of debate and controversy for histopathologists.
Objective:
To identify and specify the glycotypes of capillary endothelial cells in usual interstitial pneumonia (UIP) compared to those found in normal tissue.
Methods:
Sections of formalin-fixed, paraffin-embedded blocks from 16 cases of UIP were studied by lectin histochemistry with a panel of 27 biotinylated lectins and an avidin-peroxidase revealing system.
Results:
High expression of several classes of glycan was seen de novo in capillary endothelial cells from patients with UIP including small complex and bi/tri-antennary bisected complex N-linked sequences bolund by Concanavalin A and erythro-phytohemagglutinin, respectively, GalNAca1 residues bound by Helix pomatia and Maclura pomifera agglutinins, and L-fucosylated derivatives of type II glycan chains recognized by Ulex europaeus agglutinin-I. Glycans bound by agglutinins from Lycopersicon esculentum (β1,4GlcNAc) and Wisteria floribunda (GalNAc) as well as GlcNAc oligomers bound by Phytolacca americana and succinylated Wheat Germ agglutinin were also seen in the capillary endothelial cells of UIP. In contrast, L-fucosylated derivatives of type I glycan chains were absent in cells from cases of UIP when Anguilla anguilla agglutinin was applied, unlike the situation in normal tissue.
Conclusion:
These results may indicate existence of two distinct populations of endothelial cell in UIP with markedly different patterns of glycosylation, reflecting a pattern of differentiation and angiogenesis, which is not detectable morphologically.
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