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Updated: Aug 8, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
10:51

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Published on: July 14, 2017

Human interleukin-1 alpha is chemotactic for normal human keratinocytes

D N Sauder1, F W Orr, S Matic

  • 1Department of Medicine, McMaster University, Hamilton, Ontario, Canada.

Immunology Letters
|August 1, 1989
PubMed

Insights

Interleukin-1 (IL-1) drives keratinocyte migration by binding to specific receptors. This study confirms IL-1

Area of Science:

  • Dermatology and Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Interleukin-1 (IL-1) exhibits mitogenic and chemotactic effects on various cells, including keratinocytes.
  • Previous research suggests the presence of IL-1 receptors on keratinocytes.

Purpose of the Study:

  • To confirm the chemotactic role of IL-1 in keratinocyte migration.
  • To demonstrate and characterize IL-1 receptors on keratinocytes.

Main Methods:

  • Radio receptor assay to detect IL-1 receptors on keratinocytes.
  • Crosslinking studies using IL-1 alpha to identify receptor components.
  • Assessment of keratinocyte migration in response to IL-1.

Main Results:

  • The chemotactic effect of IL-1 on keratinocytes was confirmed.
  • IL-1 receptors were successfully demonstrated on keratinocytes.
  • Crosslinking identified two major IL-1 receptor bands at Mr 97 kDa and 133 kDa.

Conclusions:

  • Keratinocytes possess high-affinity IL-1 receptors.
  • Keratinocytes respond to IL-1 through directed migration, indicating a functional role in cellular processes.

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