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Circulating level of CD4+ CD25+ FOXP3+ T cells in patients with chronic urticaria
Saba Arshi1, Delara Babaie, Mohammad Nabavi
1Department of Allergy and Clinical Immunology, Hazrate Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran.
Insights
Chronic urticaria (CU) patients show reduced CD4+ CD25+ T-regulatory (Treg) cells. This study found lower Treg cell frequency in CU, suggesting a potential role in the condition
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- CD4+ CD25+ T-regulatory (Treg) cells are crucial for immune tolerance and preventing autoimmune diseases.
- The specific characteristics of Treg cells in chronic urticaria (CU) remain under-investigated.
- Understanding Treg cell dynamics is essential for elucidating CU pathogenesis.
Purpose of the Study:
- To quantify circulating CD4+ CD25+ FOXP3+ T cells in CU patients.
- To measure serum levels of key cytokines (IL-10, TGF-β, IL-17) in CU.
- To compare these immune markers between CU patients (autoimmune and idiopathic) and healthy controls.
Main Methods:
- Flow cytometry was used to determine the frequency of CD4+ CD25+ T cells and FOXP3 expression in peripheral blood mononuclear cells (PBMCs).
- Enzyme-linked immunosorbent assay (ELISA) quantified serum levels of IL-10, TGF-β, and IL-17.
- Peripheral blood samples were collected from CU patients and healthy individuals.
Main Results:
- A statistically significant reduction in the percentage of circulating CD4+ CD25+ FOXP3+ T cells was observed in CU patients compared to controls.
- No significant differences were found in serum levels of IL-10, TGF-β, or IL-17 between CU patients and healthy controls.
- These findings indicate a potential deficit in Treg cell numbers in CU.
Conclusions:
- The study concludes that CU patients exhibit a decreased frequency of Treg cells in their peripheral blood.
- Further research is necessary to fully understand the role of Treg cells in CU development.
- Investigating factors that regulate Treg cell function is a key area for future studies.
Background:
CD4+ CD25+ T-regulatory (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity. As characteristics of Treg cells have not been precisely investigated in chronic urticaria (CU) yet, this study was performed.
Objective:
To determine the frequencies of circulating CD4+ CD25+ FOXP3+ T cells and serum levels of interleukin (IL)-10, transforming growth factor (TGF)-β, and IL-17 in patients with chronic autoimmune urticaria and chronic idiopathic urticaria compared to healthy controls.
Methods:
Peripheral blood mononuclear cells (PBMCs) were obtained from patients with CU and healthy controls. The frequency of CD4+ CD25+ T cells in PBMCs and expression levels of FOXP3 were detected by flow cytometry. The serum levels of IL-10, TGF-β, and IL-17 were measured by enzyme-linked immunosorbent assay.
Results:
A significant decrease in the percentage of circulating CD4+ CD25+ FOXP3+ T cells was detected in patients with CU, compared to control subjects. However, no significant difference was detected on the serum levels of IL-10, TGF-β, and IL-17 between patients with CU and control subjects.
Conclusions:
This study demonstrated that the frequency of Treg cells in PBMCs was decreased in patients with CU. Further studies are needed to clarify the exact role of Treg cells in the pathogenesis of CU and factors regulating their function.
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