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Updated: Apr 17, 2026

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

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[Immunochemical study of nuclear matrix proteins localization in the structure of perinucleolar chromatin]

M I Murasheva, Iu S Chentsov

    Tsitologiia
    |February 25, 2015
    PubMed

    Insights

    Proteins from autoimmune disease patients were found in the perinucleolar chromatin of pig kidney cells. This suggests these nuclear matrix proteins are part of a chromosomal domain linked to nucleolar function.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Immunology

    Background:

    • Nuclear matrix proteins play crucial roles in nuclear structure and function.
    • Perinucleolar chromatin is a specialized region of chromatin surrounding the nucleolus.
    • Autoimmune diseases can involve autoantibodies targeting nuclear components.

    Purpose of the Study:

    • To investigate the localization of specific nuclear matrix proteins in relation to the nucleolus.
    • To determine if these proteins are associated with perinucleolar chromatin.
    • To explore the potential role of these proteins in nucleolar function.

    Main Methods:

    • Immunofluorescence labeling using antibodies from autoimmune disease patients' sera.
    • Analysis of protein localization in interphase nuclei of pig kidney cells.
    • Fractionation of cells to extract DNA, PNA, and histones to study residual structures.

    Main Results:

    • Proteins with molecular masses 27, 38, 40, 50, and 65 kDa were detected in perinucleolar chromatin.
    • These proteins were found as clusters or granules around, but not within, nucleoli.
    • After DNA and histone extraction, these proteins remained associated with residual nucleoli and karyoplasm.

    Conclusions:

    • Nuclear matrix proteins of specific molecular masses are components of perinucleolar chromatin.
    • Nucleolar protein B23 is also localized to the periphery of residual nucleoli, within the perinucleolar chromatin region.
    • Perinucleolar chromatin may represent a distinct chromosomal domain involved in nucleolar organization and function.

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