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Updated: Aug 8, 2026
Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Cytogenetic abnormalities in benign lymphoid hyperplasia: a dual-parameter study using chromosome analysis and flow
1Kanematsu Laboratories, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
Insights
Benign reactive lymphoid tissue can exhibit abnormal chromosome populations and DNA content. Further analysis combining cytogenetics and flow cytometry (FCM) is crucial for accurate identification.
Area of Science:
- Hematology
- Cytogenetics
- Immunology
Background:
- Benign reactive hyperplasia in lymphoid tissues can present with chromosomal abnormalities.
- Early detection of such abnormalities is critical, even in clinically well patients.
Purpose of the Study:
- To investigate the presence and nature of chromosomal abnormalities in benign reactive lymphoid hyperplasia.
- To evaluate the utility of cytogenetic analysis and flow cytometry (FCM) in identifying these abnormalities.
Main Methods:
- Prospective study of 20 lymphoid tissue samples (18 lymph nodes, 2 tonsils).
- Cytogenetic analysis of mitogen-stimulated cells.
- DNA content measurement using flow cytometry (FCM).
- Surface membrane immunoglobulin studies.
Main Results:
- Clonal chromosome abnormalities were detected in 9 of 20 samples.
- Abnormal DNA content by FCM was found in 10 of 20 samples.
- Monoclonal surface immunoglobulin was detected in 5 of 15 samples.
- No direct correlation found between immunoglobulin studies and cytogenetic/FCM analyses.
Conclusions:
- Aneuploidy is a common finding in reactive lymphoid tissue.
- Integrated use of cytogenetics and FCM is necessary for comprehensive identification of abnormalities.
- The study highlights the complexity of chromosomal changes in non-malignant lymphoid proliferation.
Abstract:
This is a prospective study of lymphoid tissue showing benign reactive hyperplasia (18 lymph nodes and 2 tonsils), using cytogenetic analysis of cells stimulated with T- or B-cell mitogens. The reason for this study was the detection of an abnormal chromosomal population in cells from an enlarged lymph node excised from a 7-year-old female who on further investigation was found to be clinically well and after one year's close follow-up had not developed any further signs or symptoms of malignancy. In addition, DNA content was measured by flow cytometry (FCM) on fresh cell suspensions in 17 cases and fixed cell suspensions in 3 cases. Structural and numerical clonal chromosome abnormalities were found in 9 of the 20 samples, but no common specific defect was identified. FCM showed an abnormal DNA content in 10 of the 20 samples studied; 3 of these showed clonal chromosome abnormalities. Surface membrane immunoglobulin studies were carried out on 15 of the 20 samples using cell suspensions and frozen tissue sections. In 5 of the 15 cases, monoclonal surface immunoglobulin was detected. There was no direct correlation between the surface membrane immunoglobulin studies and the chromosome and FCM analyses. We conclude that aneuploidy is a common feature in reactive lymphoid tissue, but both cytogenetics and FCM are needed to identify it.
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