Temporal arteritis: cell composition and the possible pathogenetic role of cell-mediated immunity

A biopsy specimen exhibiting the typical morphologic characteristics of temporal arteritis was studied by using light immunofluorescent, and electron microscopy and immunohistochemical techniques. The granulomatous lesion consisted of clusters of macrophages, epithelioid cells, giant cells, and the peripheral lymphocyte mantle, and was localized mainly in the media. Neutrophils were rare, and fibrinoid necrosis was absent. In immunofluorescent and immunohistochemical studies, no significant deposition of immunoglobulins or complement was observed. Immunohistochemical study with monoclonal antibodies to leukocyte surface antigens demonstrated that the central aggregated granulomatous infiltrate consisted of OKTM1+, Leu-M3+, HLA-DR+ epithelioid macrophages and multinucleated giant cells, whereas OKT8+, HLA-DR+ (suppressor/cytotoxic) T cells predominated in the peripheral lymphocyte mantle. These findings suggest that cell-mediated immunity, especially T cell-regulated granulomatous reaction, may play an important role in the pathogenesis of temporal arteritis. By electron microscopy, smooth muscle cells often exhibited closely attached macrophages, epithelioid cells, and giant cells, and displayed a variety of cell injuries. It therefore seems likely that smooth muscle cells are a primary target of the granulomatous reaction.