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[Lamin B1 and lamin B2 in human skin in the process of aging]

N N Golubtsova1, F N Filippov1, A G Gunin1

  • 1Chuvash State University, Cheboksary, 428015, Russian Federation; histol@mail.ru.

Advances in Gerontology = Uspekhi Gerontologii
|May 18, 2017
PubMed

Insights

This study investigated B type lamins in human skin, finding lamin B1 levels correlate with age-related fibroblast decline. Lamin B2 expression remained stable, suggesting lamin B1

Area of Science:

  • Cell Biology
  • Dermatology
  • Aging Research

Background:

  • Lamins are nuclear envelope proteins crucial for nuclear structure and gene regulation.
  • B type lamins (Lamin B1 and Lamin B2) are ubiquitously expressed.
  • Changes in lamin expression are implicated in aging and disease.

Purpose of the Study:

  • To investigate the expression patterns of Lamin B1 and Lamin B2 in human dermal fibroblasts across different age groups.
  • To determine the relationship between B type lamin expression and the age-dependent decrease in dermal fibroblast numbers.

Main Methods:

  • Indirect immunohistochemistry was used to detect Lamin B1 and Lamin B2 in human skin sections.
  • Quantitative analysis of positive staining in dermal fibroblasts was performed.
  • Fibroblast counts in the dermis were assessed in relation to age.

Main Results:

  • Lamin B1 positive staining in dermal fibroblasts increased with age, particularly after 40 years.
  • Lamin B2 positive staining gradually decreased from gestation to 85 years old.
  • Fibroblast numbers in the dermis significantly decreased with age, most notably from gestation to 20 years.

Conclusions:

  • Lamin B1 expression in dermal fibroblasts shows an age-dependent increase.
  • The study suggests Lamin B1 may play a role in the age-related reduction of dermal fibroblasts.
  • Lamin B2 expression is relatively stable throughout human skin aging.

Keywords:
agingfibroblastslamin B1lamin B2ontogenesisskin

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