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Updated: Mar 1, 2026
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
In-depth immunophenotyping data of IL-6R on the human peripheral regulatory T cell (Treg) compartment
Ricardo C Ferreira1,2, Daniel B Rainbow1,2, Arcadio Rubio García1,2
1JDRF/Wellcome Trust Diabetes and Inflammation Laboratory, Wellcome Trust Centre for Human Genetics, Nuffield Department of Medicine, NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.
Insights
This study characterizes human regulatory T cells (Tregs), focusing on those with high IL-6 receptor (IL-6R) expression. These Tregs exhibit distinct functional and transcriptional profiles, responding to IL-6 and IL-2 signaling.
Area of Science:
- Immunology
- Cell Biology
- T Cell Differentiation
Background:
- Regulatory T cells (Tregs) are crucial for immune homeostasis.
- The human peripheral Treg compartment is heterogeneous.
- Interleukin-6 receptor (IL-6R) expression on Tregs is not fully understood.
Purpose of the Study:
- To comprehensively characterize human peripheral CD4+ CD127lowCD25+ Tregs.
- To define the phenotype and function of Tregs expressing high levels of IL-6 receptor (IL-6R).
- To investigate the responsiveness of these Treg subsets to IL-6 and IL-2.
Main Methods:
- Flow cytometry for surface marker expression analysis.
- Transcriptional profiling to assess gene expression.
- Functional assays to evaluate suppressive capacity and cytokine responsiveness.
Main Results:
- Detailed phenotypic characterization of human peripheral Tregs.
- Identification and description of a Treg subset with high IL-6R expression.
- Demonstration of distinct transcriptional profiles and functional features for IL-6Rhi Tregs.
- Evidence of Treg subset responsiveness to IL-6 signaling in vitro and IL-2 in vivo.
Conclusions:
- The study provides a detailed characterization of human peripheral Tregs.
- A distinct Treg population with high IL-6R expression was identified and functionally assessed.
- These findings contribute to understanding Treg heterogeneity and function in immune responses.
Abstract:
We provide in this paper a detailed characterization of the human peripheral CD4+ CD127lowCD25+ regulatory T cell (Treg) compartment, with a particular emphasis in defining the population expressing higher levels of the IL-6 receptor (IL-6R). We provide a description of the phenotype of this population by assessing both the surface expression by flow cytometry as well as their transcriptional profile and functional features. In addition, we also present functional data describing the responsiveness of these subsets to IL-6 signalling in vitro and to IL-2 in vivo. The data presented in this paper support the research article "Human IL-6RhiTIGIT- CD4+CD127lowCD25+ T cells display potent in vitro suppressive capacity and a distinct Th17 profile" (Ferreira RC et al., 2017; doi: 10.1016/j.clim.2017.03.002) [1].
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