Related Experiment Video

Updated: Feb 14, 2026

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.9K

SLAMF1 is required for TLR4-mediated TRAM-TRIF-dependent signaling in human macrophages

Maria Yurchenko1,2, Astrid Skjesol3, Liv Ryan3

  • 1Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway mariia.yurchenko@ntnu.no.

The Journal of Cell Biology
|February 15, 2018
PubMed

Insights

Signaling lymphocytic activation molecule family 1 (SLAMF1) is crucial for human macrophage response to Gram-negative bacteria. It regulates Toll-like receptor 4 (TLR4) signaling by controlling TRAM trafficking to bacterial phagosomes.

Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Signaling lymphocytic activation molecule family 1 (SLAMF1) is an immune cell receptor involved in signal transduction.
  • Toll-like receptor 4 (TLR4) signaling is critical for innate immunity against bacterial pathogens.

Purpose of the Study:

  • To investigate the role of SLAMF1 in human macrophage response to Gram-negative bacteria.
  • To elucidate the molecular mechanisms by which SLAMF1 influences TLR4-mediated immunity.

Main Methods:

  • Macrophage culture and stimulation with *Escherichia coli*.
  • Immunofluorescence microscopy to track protein localization.
  • Co-immunoprecipitation assays to study protein interactions.
  • Rab11-dependent trafficking analysis.

Main Results:

  • SLAMF1 is essential for TLR4-induced interferon-beta production and bacterial killing in human macrophages.
  • SLAMF1 controls the trafficking of Toll receptor-associated molecule (TRAM) from the endocytic recycling compartment to *E. coli* phagosomes.
  • SLAMF1 interacts with TRAM via specific domains, a mechanism observed in human but not mouse cells.

Conclusions:

  • SLAMF1 plays a novel role in regulating TLR4-TRAM-TRIF inflammatory signaling in human macrophages.
  • SLAMF1 is a potential therapeutic target for modulating innate immune responses against Gram-negative bacterial infections.

More Related Videos

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF107:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

955
Author Spotlight: Optimizing Antibody-Based Cancer Treatments via Antibody-Dependent, Cell-Mediated Cytotoxicity Assay05:21

Author Spotlight: Optimizing Antibody-Based Cancer Treatments via Antibody-Dependent, Cell-Mediated Cytotoxicity Assay

Published on: September 13, 2024

923

Related Concept Videos

Contact-dependent Signaling01:19

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.7K
Requirements for Human Life01:26

Requirements for Human Life

The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
14.2K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.6K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K
Paracrine Signaling01:21

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
59.7K
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
1.8K