Related Experiment Video

Updated: Feb 7, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
08:57

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

8.3K

Production of biologically active feline interferon beta in insect larvae using a recombinant baculovirus

Mariana Bernadett Arregui1,2, Gregorio Juan Mc Callum1,2, Ignacio Smith1,2

  • 11Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina.

3 Biotech
|August 4, 2018
PubMed

Insights

Researchers developed a cost-effective method to produce recombinant feline interferon beta (rFeIFNβ) in insect larvae. This scalable production yields biologically active rFeIFNβ for research and veterinary applications.

Area of Science:

  • Biotechnology
  • Immunology
  • Virology

Background:

  • Feline interferon beta (FeIFNβ) is a type I cytokine with antitumor, antiviral, and immunomodulatory properties.
  • Efficient production of FeIFNβ is crucial for research and veterinary applications.

Purpose of the Study:

  • To establish a cost-effective platform for large-scale production of recombinant feline interferon beta (rFeIFNβ).
  • To characterize the biological activity and glycosylation of the produced rFeIFNβ.

Main Methods:

  • Recombinant feline interferon beta (rFeIFNβ) was expressed in *Spodoptera frugiperda* insect larvae using a recombinant baculovirus system.
  • Purification was performed using Blue-Sepharose chromatography from larval hemolymph.
  • Biological activity was assessed through in vitro antiviral and antitumor assays.
  • Glycosylation was confirmed via peptide-N-glycosidase F digestion.

Main Results:

  • Purified rFeIFNβ exhibited a specific activity of 1 × 106 IU mg-1.
  • A yield of 8.9 × 104 IU of rFeIFNβ per larva was achieved.
  • The rFeIFNβ demonstrated potent in vitro antiviral activity (9.5 × 104 IU mL-1) and antitumor activity comparable to commercial FeIFNω.
  • Glycosylation of rFeIFNβ was confirmed.

Conclusions:

  • A cost-effective and scalable platform for producing biologically active rFeIFNβ in insect larvae was successfully developed.
  • This method provides a valuable resource for laboratory research and veterinary medicine applications requiring feline interferon beta.

Keywords:
BaculovirusFeline interferon betaInsect larvaeVeterinary medicine

More Related Videos

Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System10:31

Process Development for the Production and Purification of Adeno-Associated Virus AAV2 Vector using Baculovirus-Insect Cell Culture System

Published on: January 13, 2022

10.4K
A Method For Production of Recombinant mCD1d Protein in Insect Cells.09:41

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

Published on: December 10, 2007

11.2K

Related Concept Videos

Osmoregulation in Insects01:47

Osmoregulation in Insects

Malpighian tubules are specialized structures found in the digestive systems of many arthropods, including most insects, that handle excretion and osmoregulation. The tubules are typically arranged in pairs and have a convoluted structure that increases their surface area.
17.6K
What is Conservation Biology?01:57

What is Conservation Biology?

Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.4K
Drug Products: Biologics, Biosimilars and Interchangeables01:28

Drug Products: Biologics, Biosimilars and Interchangeables

Body:Biologics, derived from living sources such as humans, animals, or microorganisms, represent a significant category of pharmaceuticals. These complex molecules, developed through advanced biotechnological methods or purified from natural sources, include essential medical treatments like insulin and growth hormones. The complexity of biologics arises from their large molecular structures and the intricate processes required for their production, making them distinct from conventional...
279
Biological Effects of Radiation02:59

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.0K
Recombinant DNA01:09

Recombinant DNA

Overview
103.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.8K