Related Experiment Video

Updated: Jan 26, 2026

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB
08:10

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis QDB

Published on: August 21, 2018

11.5K

Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers

Wenfeng Zhang1, Guohua Yu2, Yunyun Zhang1

  • 1Yantai Quanticision Diagnostics Inc., A Division of Quanticision Diagnostics, Inc. (US), Yantai Hi-tech District, Shandong, 264670, PR China.

Analytical Biochemistry
|April 12, 2019
PubMed

Insights

A new Quantitative Dot Blot (QDB) method simplifies tissue biomarker analysis. This method accurately quantifies breast cancer biomarkers like Estrogen Receptor (ER) and Ki67, offering an efficient alternative to ELISA.

Area of Science:

  • Biochemistry
  • Oncology
  • Biotechnology

Background:

  • Sandwich ELISA methods for tissue biomarker quantitation face limitations.
  • Developing and validating immunoassays can be resource-intensive.

Purpose of the Study:

  • To introduce and validate the Quantitative Dot Blot (QDB) method as an alternative to Sandwich ELISA.
  • To assess QDB's efficiency for absolute quantitation of key breast cancer biomarkers.

Main Methods:

  • Developed QDB method using clinically validated immunohistochemistry (IHC) antibodies.
  • Absolute quantitation of Estrogen Receptor (ER), Progesterone Receptor (PR), Ki67, and Her2 protein levels.
  • Analyzed 190 frozen breast tissue biopsies.

Main Results:

  • Successfully performed absolute quantitation of ER, PR, Ki67, and Her2.
  • QDB results were validated against existing IHC data.
  • Demonstrated QDB's capability for precise biomarker measurement.

Conclusions:

  • QDB method offers a viable alternative to Sandwich ELISA for absolute tissue biomarker quantitation.
  • QDB significantly reduces assay development effort and time.
  • This method is suitable for analyzing biomarkers such as ER, PR, Ki67, and Her2 in breast cancer tissues.

Keywords:
AbsoluteBiomarkerPrecision medicineQDBQuantificationTissue

More Related Videos

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays13:12

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Published on: April 4, 2011

18.7K
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR10:23

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

12.0K

Related Concept Videos

Mean Absolute Deviation01:13

Mean Absolute Deviation

The mean absolute deviation is also a measure of the variability of data in a sample. It is the absolute value of the average difference between the data values and the mean.
Let us consider a dataset containing the number of unsold cupcakes in five shops: 10, 15, 8, 7, and 10. Initially, calculate the sample mean. Then calculate the deviation, or the difference, between each data value and the mean. Next, the absolute values of these deviations are added and divided by the sample size to...
3.3K
Dot Product01:29

Dot Product

The dot product is an essential concept in mathematics and physics.
In engineering, the dot product of any two vectors is the product of the magnitudes of the vectors and the cosine of the angle between them. It is denoted by a dot symbol between the two vectors.
Consider a vehicle pulling an object along the ground using a rope. If the rope makes an angle with the horizontal axis, the work done can be calculated using the dot product of the force applied and the object's displacement.
The dot...
934
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
20.4K
Absolute and Local Extreme Values01:22

Absolute and Local Extreme Values

The highest and lowest values of a function, relative to a reference axis, are known as extreme values. These include absolute maximum and absolute minimum values, which represent the highest and lowest points the function reaches across its entire domain. Within a restricted portion of the function, the highest and lowest values are referred to as local maximum and local minimum values, respectively.Periodic functions, such as sine and cosine, show extreme values at infinitely many points due...
59
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
22.7K
Dot Product: Problem Solving01:21

Dot Product: Problem Solving

The dot product is a powerful tool in problem-solving involving vectors, given that the dot product of two vectors is the product of their magnitudes and the cosine of the angle between them measured anti-clockwise. Solving problems involving the dot product requires understanding its properties and developing a step-by-step process to solve them. Here are the main steps to follow when solving any general problem involving the dot product:
Identify the problem: Start by reading the problem and...
693