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Updated: Jan 26, 2026
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
[Detection and Analysis of T Lymphocyte Subsets and B Lymphocytes in Patients with Acute Leukemia]
Juan Cheng1, Hai-Zhen Ma2, Hao Zhang2
1Department of Hematology, The First Hospital of Lanzhou University, Laznhou 730000, Gansu Province, China,E-mail: chenggu029@163.com.
Insights
Peripheral blood immune cell changes, including T lymphocyte subsets, B cells, and NK cells, are significant in acute leukemia patients. Monitoring these cells aids in assessing disease severity, treatment effectiveness, and prognosis.
Area of Science:
- Immunology
- Hematology
- Oncology
Background:
- Acute leukemia is characterized by abnormal proliferation of immature white blood cells.
- Immune system dysregulation, particularly involving lymphocyte subsets, is implicated in leukemia pathogenesis and progression.
Purpose of the Study:
- To investigate dynamic changes in peripheral blood T lymphocyte subsets, B lymphocytes, and Natural Killer (NK) cells in acute leukemia patients.
- To evaluate the clinical significance of these immune cell alterations in disease severity, treatment response, and prognosis.
Main Methods:
- Flow cytometry was employed to analyze T lymphocyte subsets (CD3+, CD3+CD4+, CD3+CD8+, Treg), B lymphocytes (CD3-CD19+), and NK cells in 95 acute leukemia patients (ALL and AML) and 50 healthy controls.
- Comparisons were made between newly diagnosed patients, those in complete remission, and normal controls.
Main Results:
- Newly diagnosed acute leukemia patients exhibited significantly lower levels of CD3+, CD3+CD4+, CD3+CD8+, NK cells, and CD4+/CD8+ ratios compared to controls.
- T regulatory (Treg) cells were significantly elevated in newly diagnosed patients but decreased post-remission.
- B lymphocyte (CD3-CD19+) levels differed between ALL and AML subtypes, with distinct changes observed after remission induction.
Conclusions:
- Significant alterations in T lymphocyte subsets, B lymphocytes, and NK cells are characteristic of acute leukemia.
- Monitoring these immune cell populations provides valuable evidence for evaluating acute leukemia severity, treatment efficacy, and patient prognosis.
Objective:
To investigate the changes of T lymphocyte subsets, B lymphocyte and NK cells in peripheral blood of patients with acute leukemia at different periods and their significance.
Methods:
The peripheral T lymphocyte subsets and B lymphocyte of 95 patients with acute leukemia [(43 cases of acute lymphoblastic leukemia (ALL), 52 cases of acute myeloid leukemia (AML)] and 50 normal people were detected by flow cytometry respectively.
Results:
The positive rate of CD3+, CD3+CD4+, CD3+CD8+, NK cells and CD4+/CD8+ in the patients with newly diagnosed acute leukemia were significantly lower than those in normal controls (P<0.05) , but increased obviously after complete remission. The positive rate of Treg cells in the patients with newly diagnosed leukemia group was significantly higher than that in normal controls (P<0.01) , but decreased obviously after complete remission. Positive rate of CD3-CD19+ cells in the patients with newly diagnosed acute myeloid leukemia was significantly lower , but higher in the patients with newly diagnosed acute lymphoblastic leukemia than that in normal controls with statistical significant difference (P<0.05) , and increased obviously in the patients with acute myeloid leukemia (P<0.05) after complete remission , but decreased in the patients with acute lymphoblastic leukemia (P<0.01) after complete remission or no-remission.
Conclusion:
Changes of T lymphocyte subsets, B lymphocytes and NK cells in the patients with newly diagnosed acute leukemia are significant, thus the detection of T lymphocyte cell subsets, B lymphocytes and NK cells can provide some evidences. for evaluation of the disease severity, curative efficiency and prognosis of patients with acute leukemia.
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